APPBP1/6xHis-UBA3
APPBP1/UBA3 is the heterodimeric E1 enzyme that conjugates Nedd8 to protein substrates. Nedd8 is conjugated onto the catalytic cysteine residue of UBA3 by formation of a thioester bond in an ATP-dependent reaction. The activated Nedd8 is then passed on to an E2 (Ubc12) to be finally attached to a substrate. Nedd8 is mainly conjugated on Cullins to increase the activity of the Cullin – RING E3 Ub ligases.
Product Name:
APPBP1/6xHis-UBA3
>Also Known As:
APPBP1 (NAE1;HPP1; ula-1; A-116A10.1); UBA3 (UBE1C; hUBA3; MGC22384; DKFZp566J164)
>Catalog No.:
B1400
>Size:
25 µg
>Molecular Weight:
APPBP1 (60.2 kDa), UBA (51.8 kDa)
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~95% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
NM_003905 (APPBP1); NM_003968 (UBA3)
>Image(s):
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified APPBP1/6xHis-UBA3 |
>Shipping Method:
Dry ice shipping
>References:
1. Whitby FG, et al. (1998) J Biol Chem 273(52), 34983 – 34991.
2. Osaka F, et al. (1998) Genes Dev 12(15), 2263 – 2268.
3. Walden H, et al. (2003) Mol Cell 12(6), 1427 – 1437.
4. Schulman BA, et al. (2009) Nat Rev Mol Biol 10(5), 319 – 331. PLX8394 18474743
