Ni-NTA XPure Agarose Resin
Ni-NTA XPure Agarose Resin can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni-NTA XPure Agarose Resin consists of 90µm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni-NTA XPure Agarose Resin is highly stable and expand the range of suitable operating conditions.
Product Name:
Ni-NTA XPure Agarose Resin
>Catalog No.:
P3020-5
>Size:
5 ml
>Bead (Geometry, size):
45 – 165 µm
>Cross-Linked:
Yes
>Ligand:
Nitilotriacetic acid (NTA)
>Agarose %:
Highly cross-linked 6% agarose
>Binding Capacity:
> 40mg 6xHis-tagged protein/ml medium
>Volume %:
50% (v/v) aqueous suspension containing 20% Ethanol
>Storage Temperature:
2-8 °C
>Application:
Batch, Gravity, MPLC and FPLC
>PDF Data Sheet:
PDF Datasheet
>Users Manual:
PDF User’s Guide
>Image(s):
No
>Shipping Method:
Wet ice shipping THZ2 18473720
