TAMRA-Ub41-54(K48)-Ub
Class II fluorescence polarization assay reagent for deubiquitylating enzymes, based on the peptide sequence 41 – 54 of ubiquitin. The peptide is modified on the N-terminus with a 5carboxytetramethylrhodamine and conjugated at Lys48 to Ub via a native isopeptide bond. See ref. 4-6 for full experimental and analytical details.
Product Name:
TAMRA-Ub41-54(K48)-Ub
>Also Known As:
N/A
>Catalog No.:
M4060
>Size:
1 mg
>Molecular Weight:
10.59 kDa by MS
>Species:
Human
>Source:
Synthetic
>Stock:
in DMSO
>Concentration:
1 mg/ml
>Quality Assurance:
≥95% by RP-HPLC
>Storage:
Store powder and DMSO stock at −20°C, aqueous stocks at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
N/A
>Image(s):
N/A
>Shipping Method:
Wet ice shipping
>References:
1. Tirat et al. Anal. Biochem. 2005, 343, 244.
2. Huang et al. Methods in Molecular Biology 2009, 565, 127.
3. Levine et al. Anal. Biochem. 1997, 247, 83.
4. Faesen et al. Chem. Biol. 2011, 18, 1550.
5. Geurink et al. ChemBiochem 2012, 13, 293.
6. Mevissen et al. Cell 2013, 154, 169.
HG-9-91-01 18470936
