D allicin treatment. (A) Levels of eNOS and iNOS mRNA were determined by reverse transcription PCR following 12 h treatment with H2O2 and/or allicin as indicated. GADPH was used as an internal control. Each PCR product (5 L) was BUdR dose separated on a 1.0 agarose gel. (B) Changes in eNOS mRNA expression were verified by Duvoglustat site real-time quantitative PCR. Values represent mean ?SD from three samples per group; #p < 0.05 compared with normal (untreated) HUVECs; *p < 0.05 compared with model (H2O2 only) group.elucidate a pathway by which protection is mediated via the reduction in oxidative stress.QY and CR obtained copies of the studies and revised the writing. All the authors read and approved the final manuscript. Acknowledgments This work was supported by Zhejiang Provincial Natural Science Foundation of China (LQ13H310003, LY13H020007, LY14H290003), the Research Foundation of Education Bureau of Zhejiang Province, China (Y20096333, Y200906336), and the fund of Zhejiang province for medical sciences (2009B114). Author details 1 College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. 2Hangzhou pharmavaxin co., LTD, Hangzhou 310052, China. 3 The First Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310006, China. Received: 17 July 2014 Accepted: 27 August 2014 Published: 30 August 2014 References 1. Chan JY, Yuen AC, Chan RY, Chan SW: A review of the cardiovascular benefits and antioxidant properties of allicin. Phytother Res 2013, 27(5):637?46. 2. Lusis AJ: Atherosclerosis. Nature 2000, 407(6801):233?41. 3. Libby P: Inflammation in atherosclerosis. Nature 2002, 420(6917):868?74.Conclusion Allicin has powerful effects in protecting HUVECs from apoptosis. The protection occurs via a mechanism involving the reduction in oxidative stress, as measured by increased SOD and reduced MDA, NO and eNOS. There finding suggest that allicin functions as a powerful antioxidant. Further studies will be necessary to determine the direct effects of allicin on atherosclerosis.Competing interests The authors declare that they have no competing interest regarding the publication of this article. Authors' contributions CH, WL and ZL developed the idea and designed the research. CS and TY wrote the manuscript, selected which studies to include and extracted the data from the studies, interpreted the analysis and drafted the final review.Chen et al. BMC Complementary and Alternative Medicine 2014, 14:321 http://www.biomedcentral.com/1472-6882/14/Page 8 of4.5. 6.7.8.9.10.11.12.13.14.15. 16.17.18. 19. 20.21.22. 23.24.25. 26.27.Herman AG, Moncada S: Therapeutic potential of nitric oxide donors in the prevention and treatment of atherosclerosis. Eur Heart J 2005, 26(19):1945?955. Osterud B, Bjorklid E: Role of monocytes in atherogenesis. Physiol Rev 2003, 83(4):1069?112. Wang YK, Hong YJ, Wei M, Wu Y, Huang ZQ, Chen RZ, Chen HZ: Curculigoside attenuates human umbilical vein endothelial cell injury induced by H2O2. J Ethnopharmacol 2010, 132(1):233?39. Roberto D, Micucci P, Sebastian T, Graciela F, Anesini C: Antioxidant activity of limonene on normal murine lymphocytes: relation to H2O2 modulation and cell PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27362935 proliferation. Basic Clin Pharmacol Toxicol 2010, 106(1):38?4. Arzanlou M, Bohlooli S, Jannati E, Mirzanejad-Asl H: Allicin from garlic neutralizes the hemolytic activity of intra- and extra-cellular pneumolysin O in vitro. Toxicon 2011, 57(4):540?45. Eilat S, Oestraicher Y, Rabinkov A, Ohad D, Mirelman D, Battler A, Eldar M.D allicin treatment. (A) Levels of eNOS and iNOS mRNA were determined by reverse transcription PCR following 12 h treatment with H2O2 and/or allicin as indicated. GADPH was used as an internal control. Each PCR product (5 L) was separated on a 1.0 agarose gel. (B) Changes in eNOS mRNA expression were verified by real-time quantitative PCR. Values represent mean ?SD from three samples per group; #p < 0.05 compared with normal (untreated) HUVECs; *p < 0.05 compared with model (H2O2 only) group.elucidate a pathway by which protection is mediated via the reduction in oxidative stress.QY and CR obtained copies of the studies and revised the writing. All the authors read and approved the final manuscript. Acknowledgments This work was supported by Zhejiang Provincial Natural Science Foundation of China (LQ13H310003, LY13H020007, LY14H290003), the Research Foundation of Education Bureau of Zhejiang Province, China (Y20096333, Y200906336), and the fund of Zhejiang province for medical sciences (2009B114). Author details 1 College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. 2Hangzhou pharmavaxin co., LTD, Hangzhou 310052, China. 3 The First Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310006, China. Received: 17 July 2014 Accepted: 27 August 2014 Published: 30 August 2014 References 1. Chan JY, Yuen AC, Chan RY, Chan SW: A review of the cardiovascular benefits and antioxidant properties of allicin. Phytother Res 2013, 27(5):637?46. 2. Lusis AJ: Atherosclerosis. Nature 2000, 407(6801):233?41. 3. Libby P: Inflammation in atherosclerosis. Nature 2002, 420(6917):868?74.Conclusion Allicin has powerful effects in protecting HUVECs from apoptosis. The protection occurs via a mechanism involving the reduction in oxidative stress, as measured by increased SOD and reduced MDA, NO and eNOS. There finding suggest that allicin functions as a powerful antioxidant. Further studies will be necessary to determine the direct effects of allicin on atherosclerosis.Competing interests The authors declare that they have no competing interest regarding the publication of this article. Authors' contributions CH, WL and ZL developed the idea and designed the research. CS and TY wrote the manuscript, selected which studies to include and extracted the data from the studies, interpreted the analysis and drafted the final review.Chen et al. BMC Complementary and Alternative Medicine 2014, 14:321 http://www.biomedcentral.com/1472-6882/14/Page 8 of4.5. 6.7.8.9.10.11.12.13.14.15. 16.17.18. 19. 20.21.22. 23.24.25. 26.27.Herman AG, Moncada S: Therapeutic potential of nitric oxide donors in the prevention and treatment of atherosclerosis. Eur Heart J 2005, 26(19):1945?955. Osterud B, Bjorklid E: Role of monocytes in atherogenesis. Physiol Rev 2003, 83(4):1069?112. Wang YK, Hong YJ, Wei M, Wu Y, Huang ZQ, Chen RZ, Chen HZ: Curculigoside attenuates human umbilical vein endothelial cell injury induced by H2O2. J Ethnopharmacol 2010, 132(1):233?39. Roberto D, Micucci P, Sebastian T, Graciela F, Anesini C: Antioxidant activity of limonene on normal murine lymphocytes: relation to H2O2 modulation and cell PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27362935 proliferation. Basic Clin Pharmacol Toxicol 2010, 106(1):38?4. Arzanlou M, Bohlooli S, Jannati E, Mirzanejad-Asl H: Allicin from garlic neutralizes the hemolytic activity of intra- and extra-cellular pneumolysin O in vitro. Toxicon 2011, 57(4):540?45. Eilat S, Oestraicher Y, Rabinkov A, Ohad D, Mirelman D, Battler A, Eldar M.
