Ced tumor-like characteristics by increasing the colony formation, migration, and invasion of CRC cells. Accordingly, the opposite results were obtained when TUG1 was knocked down. These results indicate that TUG1 might play a key role in promoting metastasis of CRC, which was further proven by a mice liver metastasis model in which TUG1 overexpression significantly increased the number of metastatic tumor nodules in the liver. Our study is consistent with previous research revealing that the high expression of TUG1 in primary CRC was strongly associated with lungmetastases [17]. Moreover, our data showed that high TUG1 expression in CRC tissues was closely associated with a decreased survival time in CRC patients. These multivariate analyses suggested that TUG1 might be an independent risk factor for CRC metastasis. Knowledge of how lncRNAs are regulated in complex gene regulatory systems has attracted a lot of attention. Previously, hypermethylation of the promoter or the intergenic differentially methylated region has been found to contribute to reduced expression of lncRNA MEG3 in tumors, indicating that epigenetic regulation is also involved in the expression of these genes [18]. The fact that MS023MedChemExpress MS023 whether histone deacetylation that functioned as epigenetic regulatory factors PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28893839 manipulate the expression of TGU1 remains unknown. Our findings emphasizeSun et al. J Transl Med (2016) 14:Page 7 ofFig. 3 Necrosulfonamide supplier Enhanced metastasis of CRC cells with overexpressed TUG1. a Representative image and number statistics for colony formation in SW480pcDNA and SW480pcDNA-TUG1 cells. b Wound-healing assay for motility of SW480pcDNA and SW480pcDNA-TUG1 cells. Representative pictures of one field at the beginning (t = 0) (left panel) and at the end of the recording (t = 12 h) (right panel) in each condition. c Representative images of transwell migrated cells and d invaded cells in stably transfected SW480pcDNA and SW480pcDNA-TUG1 cells and average number of migrated cells and invaded cells are shown in the right of (c) and (d). Values represent mean ?SD. * P < 0.05 compared with pcDNASun et al. J Transl Med (2016) 14:Page 8 ofFig. 4 Silenced TUG1 inhibited metastasis of CRC cells. a Representative image and number statistics for colony formation in LOVOsi-control and LOVOsi-TUG1 cells. b Wound-healing assay for motility of LOVOsi-control and LOVOsi-TUG1 cells. Representative pictures of one field at the beginning (t = 0) (left panel) and at the end (t = 12 h) (right panel) of the recording in each condition are shown. c Representative images of transwell migrated cells, and d invaded cells in stably transfected LOVOsi-control and LOVOsi-TUG1 cells and the average number of migrated cells and invaded cells are shown in the right of (c) and (d). Values represent mean ?SD. * P < 0.05 compared with si-controlthat histone deacetylase is a key factor in controlling the expression of the lncRNA TUG1. We observed that both TSA (an inhibitor for histone deacetylase) and HDACsknockdown enhanced THG1 expression. These results, along with those from a recent study [19], highlight the role of epigenetics in regulating lncRNA transcription.Sun et al. J Transl Med (2016) 14:Page 9 ofFig. 5 Statistics for mice metastatic nodules in vivo. Nude mice were injected with SW480pcDNA or SW480pcDNA-TUG1 cells and tumor nodules were numbered 7 days post-transplantation. Values represent mean ?SD. * P < 0.05 compared with SW480-controlImportant hallmarks of EMT include the loss of E-cadhe.Ced tumor-like characteristics by increasing the colony formation, migration, and invasion of CRC cells. Accordingly, the opposite results were obtained when TUG1 was knocked down. These results indicate that TUG1 might play a key role in promoting metastasis of CRC, which was further proven by a mice liver metastasis model in which TUG1 overexpression significantly increased the number of metastatic tumor nodules in the liver. Our study is consistent with previous research revealing that the high expression of TUG1 in primary CRC was strongly associated with lungmetastases [17]. Moreover, our data showed that high TUG1 expression in CRC tissues was closely associated with a decreased survival time in CRC patients. These multivariate analyses suggested that TUG1 might be an independent risk factor for CRC metastasis. Knowledge of how lncRNAs are regulated in complex gene regulatory systems has attracted a lot of attention. Previously, hypermethylation of the promoter or the intergenic differentially methylated region has been found to contribute to reduced expression of lncRNA MEG3 in tumors, indicating that epigenetic regulation is also involved in the expression of these genes [18]. The fact that whether histone deacetylation that functioned as epigenetic regulatory factors PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28893839 manipulate the expression of TGU1 remains unknown. Our findings emphasizeSun et al. J Transl Med (2016) 14:Page 7 ofFig. 3 Enhanced metastasis of CRC cells with overexpressed TUG1. a Representative image and number statistics for colony formation in SW480pcDNA and SW480pcDNA-TUG1 cells. b Wound-healing assay for motility of SW480pcDNA and SW480pcDNA-TUG1 cells. Representative pictures of one field at the beginning (t = 0) (left panel) and at the end of the recording (t = 12 h) (right panel) in each condition. c Representative images of transwell migrated cells and d invaded cells in stably transfected SW480pcDNA and SW480pcDNA-TUG1 cells and average number of migrated cells and invaded cells are shown in the right of (c) and (d). Values represent mean ?SD. * P < 0.05 compared with pcDNASun et al. J Transl Med (2016) 14:Page 8 ofFig. 4 Silenced TUG1 inhibited metastasis of CRC cells. a Representative image and number statistics for colony formation in LOVOsi-control and LOVOsi-TUG1 cells. b Wound-healing assay for motility of LOVOsi-control and LOVOsi-TUG1 cells. Representative pictures of one field at the beginning (t = 0) (left panel) and at the end (t = 12 h) (right panel) of the recording in each condition are shown. c Representative images of transwell migrated cells, and d invaded cells in stably transfected LOVOsi-control and LOVOsi-TUG1 cells and the average number of migrated cells and invaded cells are shown in the right of (c) and (d). Values represent mean ?SD. * P < 0.05 compared with si-controlthat histone deacetylase is a key factor in controlling the expression of the lncRNA TUG1. We observed that both TSA (an inhibitor for histone deacetylase) and HDACsknockdown enhanced THG1 expression. These results, along with those from a recent study [19], highlight the role of epigenetics in regulating lncRNA transcription.Sun et al. J Transl Med (2016) 14:Page 9 ofFig. 5 Statistics for mice metastatic nodules in vivo. Nude mice were injected with SW480pcDNA or SW480pcDNA-TUG1 cells and tumor nodules were numbered 7 days post-transplantation. Values represent mean ?SD. * P < 0.05 compared with SW480-controlImportant hallmarks of EMT include the loss of E-cadhe.
