Natural logarithm scale of OR was also used to evaluate the publication biases [35]. All the P values were two-sided. All analyses were calculated using STATA Version 12.0 software (Stata Corp, College Station, TX).Quality scores27 231G.C rs9904341 (G/C) Survivin Blood PCR-RFLP231G.C231G.C231G.C231G.C231G.C231G.C231G.CAlias namers9904341 (G/C)rs9904341 (G/C)rs9904341 (G/C)rs9904341 (G/C)rs9904341 (G/C)rs9904341 (G/C)rs9904341 (G/C)SurvivinSurvivinSurvivinSurvivinGenotype methodSurvivinSurvivinSurvivinPCR-RFLPPCR-RFLPPCR-RFLPPCR-RFLPPCR-RFLPPCR-RFLPSampleTissueTissueTaqmanBloodBloodBloodBloodBloodBloodPCR-SSCPSurvivinGeners9904341 (G/C)SNP231G.CResults The Characteristics of Included StudiesAccording to the inclusion criteria, 9 studies [21,22,24,25,28?30,36,37] were included and 36 were excluded in this metaanalysis. The flow chart of study selection is shown in Figure 1. The total of GIT cancer cases and healthy controls were 2,231 and 2,287, respectively, in these 9 case-control studies. The publication year of involved studies ranged from 2008 to 2011. All patients diagnosed with GIT cancer were also confirmed by pathological examination. Three studies used hospital-based controls, while the other six studies used population-based controls (community populations). All the studies used blood samples for genotyping except for two studies [21,22] which used tissue samples. A classical polymerase chain reaction-restriction fragment length Emixustat (hydrochloride) web polymorphism (MedChemExpress AVP PCR-RELP) method was performed in seven of the nine studies. Out of the other two studies, one study used Taqman assay and the other used polymerase chain reactionsingle strand conformation polymorphism (PCR-SSCP). Overall, there were four gastric cancer studies, three colorectal cancer studies and two esophageal cancer studies. Six of these studies were conducted in Asian populations and three in Caucasian populations. HWE test was conducted on the genotype distribution of the controls in all nine studies. Each study did not deviate from the HWE (all P.0.05). All quality scores of included studies were higher than 20 (moderate-high quality). The characteristics of 1655472 the included studies are summarized in Table 1. The genotype distribution of survivin 231G.C polymorphism is presented in Table 2.Esophageal cancerColorectal cancerColorectal cancerColorectal cancerEsophageal cancer 250 250 HB Asian 2011 India PBGastric cancerGastric cancerControlGastric cancerNumberCaseTable 1. Characteristics of included studies in this meta-analysis.ControlHBHBPBPBSourceCasePBPBHBHBHBHBHBEthnicityHBHBCaucasianCaucasianAsianAsianAsianAsianCountryAsianGreeceChinaChinaChinaChinaChinaGreeceYearBrazilCaucasianHBHBPBGastric cancerCancer typeQuantitative Data SynthesisA summary of the meta-analysis findings of the correlation between survivin 231G.C polymorphism and GIT cancer risk is provided in Table 3. The heterogeneity was significant under all genetic models (all P,0.05), which might be a result of the difference in cancer types, ethnicity, country, source of controls and genotype methods, so random effects model was used. The meta-analysis results showed that survivin 231G.C polymorphism was associated with increased risk of GIT cancers under allFirst author [Ref]Gazouli et al [22]Antonacopoulou et al [29]Yang et al-1 [25]Yang et al-2 [37]Huang et al [30]Cheng et al [21]Zhu et al [28]Borges Bdo et al [24]Upadhyay et al [36]Survivin Gene and Gastrointestinal Tract CancerTable 2. The genotype distribution of sur.Natural logarithm scale of OR was also used to evaluate the publication biases [35]. All the P values were two-sided. All analyses were calculated using STATA Version 12.0 software (Stata Corp, College Station, TX).Quality scores27 231G.C rs9904341 (G/C) Survivin Blood PCR-RFLP231G.C231G.C231G.C231G.C231G.C231G.C231G.CAlias namers9904341 (G/C)rs9904341 (G/C)rs9904341 (G/C)rs9904341 (G/C)rs9904341 (G/C)rs9904341 (G/C)rs9904341 (G/C)SurvivinSurvivinSurvivinSurvivinGenotype methodSurvivinSurvivinSurvivinPCR-RFLPPCR-RFLPPCR-RFLPPCR-RFLPPCR-RFLPPCR-RFLPSampleTissueTissueTaqmanBloodBloodBloodBloodBloodBloodPCR-SSCPSurvivinGeners9904341 (G/C)SNP231G.CResults The Characteristics of Included StudiesAccording to the inclusion criteria, 9 studies [21,22,24,25,28?30,36,37] were included and 36 were excluded in this metaanalysis. The flow chart of study selection is shown in Figure 1. The total of GIT cancer cases and healthy controls were 2,231 and 2,287, respectively, in these 9 case-control studies. The publication year of involved studies ranged from 2008 to 2011. All patients diagnosed with GIT cancer were also confirmed by pathological examination. Three studies used hospital-based controls, while the other six studies used population-based controls (community populations). All the studies used blood samples for genotyping except for two studies [21,22] which used tissue samples. A classical polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) method was performed in seven of the nine studies. Out of the other two studies, one study used Taqman assay and the other used polymerase chain reactionsingle strand conformation polymorphism (PCR-SSCP). Overall, there were four gastric cancer studies, three colorectal cancer studies and two esophageal cancer studies. Six of these studies were conducted in Asian populations and three in Caucasian populations. HWE test was conducted on the genotype distribution of the controls in all nine studies. Each study did not deviate from the HWE (all P.0.05). All quality scores of included studies were higher than 20 (moderate-high quality). The characteristics of 1655472 the included studies are summarized in Table 1. The genotype distribution of survivin 231G.C polymorphism is presented in Table 2.Esophageal cancerColorectal cancerColorectal cancerColorectal cancerEsophageal cancer 250 250 HB Asian 2011 India PBGastric cancerGastric cancerControlGastric cancerNumberCaseTable 1. Characteristics of included studies in this meta-analysis.ControlHBHBPBPBSourceCasePBPBHBHBHBHBHBEthnicityHBHBCaucasianCaucasianAsianAsianAsianAsianCountryAsianGreeceChinaChinaChinaChinaChinaGreeceYearBrazilCaucasianHBHBPBGastric cancerCancer typeQuantitative Data SynthesisA summary of the meta-analysis findings of the correlation between survivin 231G.C polymorphism and GIT cancer risk is provided in Table 3. The heterogeneity was significant under all genetic models (all P,0.05), which might be a result of the difference in cancer types, ethnicity, country, source of controls and genotype methods, so random effects model was used. The meta-analysis results showed that survivin 231G.C polymorphism was associated with increased risk of GIT cancers under allFirst author [Ref]Gazouli et al [22]Antonacopoulou et al [29]Yang et al-1 [25]Yang et al-2 [37]Huang et al [30]Cheng et al [21]Zhu et al [28]Borges Bdo et al [24]Upadhyay et al [36]Survivin Gene and Gastrointestinal Tract CancerTable 2. The genotype distribution of sur.
