Ptin, but lower levels of adiponectin, E2, and 1,25(OH)2D3. However, free E2 level was not significantly different between the two groups.Clinical characteristics and MedChemExpress CI 1011 biochemical variables in subjects with various number of MetS componentsThe clinical 22948146 characteristics and biochemical data of subjects with various numbers of MetS components are summarized in Table 2. As the number of MetS components increased, age, BMI, and leptin level increased, but adiponectin, E2, and 1,25(OH)2D3 levels decreased significantly (P,0.001 for all LY-2409021 web comparisons). Furthermore, as the number of MetS components increased, adiponectin and 1,25(OH)2D3 decreased but leptin levels increased in a linear shape. Only E2 levels decreased in a ladder shape as the numbers of MetS components increased. (Figure 1)Biochemical analysis using radioimmunoassayPeripheral venous blood samples after more than 8 hours fasting overnight were drawn into pyrogen-free tubes for analysis of serum glucose, lipid panels, and routine biochemical profiles. Through radioimmunoassay (RIA), E2 was measured using the Siemens’ RIA kits (Los Angeles, USA; Inter-assay coefficient of variation (CV): 4.0 ,7.0 ; Inter-assay CV: 4.2 ,8.1 ), while 1,25(OH)2D3 was determined using the DiaSorin RIA kits (Northwestern Avenue Stillwater, USA; Intra-assay CV: 6.8 ,11.3 ; Inter-assay CV:12.3 ,15.3 ). Leptin and adiponectin were measured using Millipore’s RIA kits (Missouri, USA; Intra-assay CV: 3.4 ,8.3 and 1.78 ,6.21 respectively; Inter-assay CV: 3.0 ,6.2 and 6.90 ,9.25 respectively). SHBG levels (detectable range: 0.2?80 nmol/L; inter-assay CV of 4.8 , and intra-assay CV of 3.5 ) were determined using a DPC Immulite analyzer (Diamond Diagnostics, Holliston, MA).Correlations between biochemical variables and individual MetS componentsAdiponectin was correlated with WC (r = 0.57, P,0.001), HDL (r = 0.46, P,0.001) and TG (r = 20.30, P,0.001). Leptin was correlated with HDL (r = 20.27, P,0.001), WC (r = 0.63, P,0.001), and DBP (r = 0.28, P,0.001); E2 level was correlated with WC (r = 20.13, P = 0.001), FBG (r = 20.16, P,0.001), TG (r = 20.11, P = 0.006), and HDL (r = 20.20, P,0.001). 1,25(OH)2D3 was correlated with WC (r = 20.12, P = 0.003),Low Estradiol and Metabolic SyndromeTable 1. Means 6 standard deviations of the baseline characteristics and biochemical variables in the subjects with and without MetS.Subjects without MetS (n = 412) Age (yrs) BMI (Kg/m2) Waist circumference (cm) Hip circumference (cm) Waist-hip ratio SBP (mm-Hg) DBP (mm-Hg) DM n ( ) Hypertension, n ( ) Dyslipidemia history, n ( ) CVD history, n ( ) Smoking, n ( ) Nonsmokers Former Smokers Current Smokers Drinking, n ( ) Non-drinker Former Drinkers Current Drinkers Betel quid, n ( ) Never Chewed Former Chewers Current Chewers Blood Biochemistry Fasting glucose (mg/dl) TG (mg/L) Total cholesterol (mg/dl) HDL-C (mg/dl) Adiponectin (ng/ml) Leptin (ng/ml) 1,25(OH)2D3 (pg/ml) E2 (pg/ml) Free E2 (pg/ml) SHBG(nmol/L) Albumin(g/dl) 94.0614.1 108.6685.0 189.5633.9 51.6611.0 14.366.9 3.361.9 47.7619.2 26.368.1 0.760.3 49.7624.7 4.460.2 404 (98.1) 6 (1.5) 1 (0.2) 356 (86.4) 6 (1.5) 50 (12.1) 315 (76.5) 57 (13.8) 40 (9.7) 55.163.6 24.462.3 83.465.6 95.366.7 0.9661.83 128.4611.8 80.568.2 16 (3.9) 71 (17.2) 40 (9.7) 16 (3.9)Subjects with MetS (n = 243) 56.765.8 27.669.1 90.666.8 106.8686.8 0.9160.08 136.5610.8 86.368.5 47 (19.3) 115 (47.3) 89 (36.6) 29 
(11.9)P value,0.001* ,0.001* ,0.001* 0.039* 0.21 ,0.001* ,0.001* ,0.001* ,0.001* ,0.001* ,0.001*.Ptin, but lower levels of adiponectin, E2, and 1,25(OH)2D3. However, free E2 level was not significantly different between the two groups.Clinical characteristics and biochemical variables in subjects with various number of MetS componentsThe clinical 
22948146 characteristics and biochemical data of subjects with various numbers of MetS components are summarized in Table 2. As the number of MetS components increased, age, BMI, and leptin level increased, but adiponectin, E2, and 1,25(OH)2D3 levels decreased significantly (P,0.001 for all comparisons). Furthermore, as the number of MetS components increased, adiponectin and 1,25(OH)2D3 decreased but leptin levels increased in a linear shape. Only E2 levels decreased in a ladder shape as the numbers of MetS components increased. (Figure 1)Biochemical analysis using radioimmunoassayPeripheral venous blood samples after more than 8 hours fasting overnight were drawn into pyrogen-free tubes for analysis of serum glucose, lipid panels, and routine biochemical profiles. Through radioimmunoassay (RIA), E2 was measured using the Siemens’ RIA kits (Los Angeles, USA; Inter-assay coefficient of variation (CV): 4.0 ,7.0 ; Inter-assay CV: 4.2 ,8.1 ), while 1,25(OH)2D3 was determined using the DiaSorin RIA kits (Northwestern Avenue Stillwater, USA; Intra-assay CV: 6.8 ,11.3 ; Inter-assay CV:12.3 ,15.3 ). Leptin and adiponectin were measured using Millipore’s RIA kits (Missouri, USA; Intra-assay CV: 3.4 ,8.3 and 1.78 ,6.21 respectively; Inter-assay CV: 3.0 ,6.2 and 6.90 ,9.25 respectively). SHBG levels (detectable range: 0.2?80 nmol/L; inter-assay CV of 4.8 , and intra-assay CV of 3.5 ) were determined using a DPC Immulite analyzer (Diamond Diagnostics, Holliston, MA).Correlations between biochemical variables and individual MetS componentsAdiponectin was correlated with WC (r = 0.57, P,0.001), HDL (r = 0.46, P,0.001) and TG (r = 20.30, P,0.001). Leptin was correlated with HDL (r = 20.27, P,0.001), WC (r = 0.63, P,0.001), and DBP (r = 0.28, P,0.001); E2 level was correlated with WC (r = 20.13, P = 0.001), FBG (r = 20.16, P,0.001), TG (r = 20.11, P = 0.006), and HDL (r = 20.20, P,0.001). 1,25(OH)2D3 was correlated with WC (r = 20.12, P = 0.003),Low Estradiol and Metabolic SyndromeTable 1. Means 6 standard deviations of the baseline characteristics and biochemical variables in the subjects with and without MetS.Subjects without MetS (n = 412) Age (yrs) BMI (Kg/m2) Waist circumference (cm) Hip circumference (cm) Waist-hip ratio SBP (mm-Hg) DBP (mm-Hg) DM n ( ) Hypertension, n ( ) Dyslipidemia history, n ( ) CVD history, n ( ) Smoking, n ( ) Nonsmokers Former Smokers Current Smokers Drinking, n ( ) Non-drinker Former Drinkers Current Drinkers Betel quid, n ( ) Never Chewed Former Chewers Current Chewers Blood Biochemistry Fasting glucose (mg/dl) TG (mg/L) Total cholesterol (mg/dl) HDL-C (mg/dl) Adiponectin (ng/ml) Leptin (ng/ml) 1,25(OH)2D3 (pg/ml) E2 (pg/ml) Free E2 (pg/ml) SHBG(nmol/L) Albumin(g/dl) 94.0614.1 108.6685.0 189.5633.9 51.6611.0 14.366.9 3.361.9 47.7619.2 26.368.1 0.760.3 49.7624.7 4.460.2 404 (98.1) 6 (1.5) 1 (0.2) 356 (86.4) 6 (1.5) 50 (12.1) 315 (76.5) 57 (13.8) 40 (9.7) 55.163.6 24.462.3 83.465.6 95.366.7 0.9661.83 128.4611.8 80.568.2 16 (3.9) 71 (17.2) 40 (9.7) 16 (3.9)Subjects with MetS (n = 243) 56.765.8 27.669.1 90.666.8 106.8686.8 0.9160.08 136.5610.8 86.368.5 47 (19.3) 115 (47.3) 89 (36.6) 29 (11.9)P value,0.001* ,0.001* ,0.001* 0.039* 0.21 ,0.001* ,0.001* ,0.001* ,0.001* ,0.001* ,0.001*.
