J Exp Psychol 53: 6276. 42. Nudo RJ, Plautz EJ, Frost SB Role of adaptive plasticity in recovery of function after damage to motor cortex. Muscle Nerve 24: 10001019. 43. Bohotin CR, Badescu M, Popescu DN, Bohotin V Motor cortex plasticityfrom physiology to clinical neurology. Rom J Physiol 41: 99108. 44. Adkins DL, Hsu JE, Jones TA Motor cortical stimulation promotes synaptic plasticity and behavioral improvements following sensorimotor cortex lesions. Exp Neurol 212: 1428. 45. Cui X, Chopp M, Zacharek A, Roberts C, Buller B, et al. Niacin treatment of stroke increases synaptic plasticity and axon growth in rats. Stroke 41: 20442049. 46. Chen J, Li Y, Wang L, Lu M, Chopp M Caspase inhibition by Z-VAD increases the survival of grafted bone marrow cells and improves functional outcome after MCAo in rats. J Neurol Sci 199: 1724. 47. Stroemer RP, Kent TA, Hulsebosch CE Enhanced neocortical neural sprouting, synaptogenesis, and behavioral recovery with D-amphetamine therapy after neocortical infarction in rats. Stroke 29: 23812393; discussion 2393-2385. 9 ~~ ~~ South Africa has a rapidly developing abalone aquaculture industry, based on the cultivation of Haliotis midae. However, the relatively slow growth rate of abalone represents a major constraint on the aquaculture industry. The use of probiotic microorganisms is becoming increasingly accepted as a means of improving the health and growth of aquacultured species. Macey and Coyne demonstrated that H. midae fed a high protein diet supplemented with the probiotic Vibrio midae SY9 had increased digestive tract protease levels, enhanced protein digestion and increased growth rates in comparison to animals fed an un-supplemented diet. Several possible modes of action have been proposed for probiotic effects observed within aquaculture environments, including the production and secretion of extracellular hydrolytic CB-5083 chemical information enzymes that contribute to, and improve, the digestion efficiency of the host. Several studies have demonstrated the effect of probiotic supplementation on abalone digestive enzyme activity levels and/or growth, and have suggested a possible role for `nutritional probiotics’ in abalone aquaculture. Abalone possess a unique microbiota that is capable of producing extracellular enzymes which degrade the major constituents of abalone feeds. However, less than 10% of the microorganisms associated with the abalone digestive tract can be cultured in the laboratory. Consequently, culture-independent methodologies are necessary for investigating gut microorganisms within their natural habitat. In situ hybridization using specific 16S rDNA oligonucleotide probes is a cultureindependent method used for investigating bacterial population diversity, and is an ideal method for investigating microorganisms in vivo. ISH techniques have been successfully used to investigate the microbiota of goldfish, abalone, Artemia nauplii and salmon, and to specifically localise 12926553 intracellular prokaryotes in abalone tissue sections. Rengpipat et al. successfully tagged the shrimp probiotiont Bacillus S11 with GFP and then monitored the presence of this probiotic within the digestive tract of the Black Tiger shrimp Penaeus monodon following dietary supplementation. Histological analysis of intestinal samples revealed that the GFP-tagged probiotic bacterium was viable and localised to the P7C3 cost surface of the shrimp’s intestine. Probiotic and Protease Localisation in Abalone Gut Macey and Coyne observed significantly i.J Exp Psychol 53: 6276. 42. Nudo RJ, Plautz EJ, Frost SB Role of adaptive plasticity in recovery of function after damage to motor cortex. Muscle Nerve 24: 10001019. 43. Bohotin CR, Badescu M, Popescu DN, Bohotin V Motor cortex plasticityfrom physiology to clinical neurology. Rom J Physiol 41: 99108. 44. Adkins DL, Hsu JE, Jones TA Motor cortical stimulation promotes synaptic plasticity and behavioral improvements following sensorimotor cortex lesions. Exp Neurol 212: 1428. 45. Cui X, Chopp M, Zacharek A, Roberts C, Buller B, et al. Niacin treatment of stroke increases synaptic plasticity and axon growth in rats. Stroke 41: 20442049. 46. Chen J, Li Y, Wang L, Lu M, Chopp M Caspase inhibition by Z-VAD increases the survival of grafted bone marrow cells and improves functional outcome after MCAo in rats. J Neurol Sci 199: 1724. 47. Stroemer RP, Kent TA, Hulsebosch CE Enhanced neocortical neural sprouting, synaptogenesis, and behavioral recovery with D-amphetamine therapy after neocortical infarction in rats. Stroke 29: 23812393; discussion 2393-2385. 9 ~~ ~~ South Africa has a rapidly developing abalone aquaculture industry, based on the cultivation of Haliotis midae. However, the relatively slow growth rate of abalone represents a major constraint on the aquaculture industry. The use of probiotic microorganisms is becoming increasingly accepted as a means of improving the health and growth of aquacultured species. Macey and Coyne demonstrated that H. midae fed a high protein diet supplemented with the probiotic Vibrio midae SY9 had increased digestive tract protease levels, enhanced protein digestion and increased growth rates in comparison to animals fed an un-supplemented diet. Several possible modes of action have been proposed for probiotic effects observed within aquaculture environments, including the production and secretion of extracellular hydrolytic enzymes that contribute to, and improve, the digestion efficiency of the host. Several studies have demonstrated the effect of probiotic supplementation on abalone digestive enzyme activity levels and/or growth, and have suggested a possible role for `nutritional probiotics’ in abalone aquaculture. Abalone possess a unique microbiota that is capable of producing extracellular enzymes which degrade the major constituents of abalone feeds. However, less than 10% of the microorganisms associated with the abalone digestive tract can be cultured in the laboratory. Consequently, culture-independent methodologies are necessary for investigating gut microorganisms within their natural habitat. In situ hybridization using specific 16S rDNA oligonucleotide probes is a cultureindependent method used for investigating bacterial population diversity, and is an ideal method for investigating microorganisms in vivo. ISH techniques have been successfully used to investigate the microbiota of goldfish, abalone, Artemia nauplii and salmon, and to specifically localise 12926553 intracellular prokaryotes in abalone tissue sections. Rengpipat et al. successfully tagged the shrimp probiotiont Bacillus S11 with GFP and then monitored the presence of this probiotic within the digestive tract of the Black Tiger shrimp Penaeus monodon following dietary supplementation. Histological analysis of intestinal samples revealed that the GFP-tagged probiotic bacterium was viable and localised to the surface of the shrimp’s intestine. Probiotic and Protease Localisation in Abalone Gut Macey and Coyne observed significantly i.
