K63 polyUb Chain-binding Protein Identification / Validation Kit
This kit is designed for two purposes: 1) identification of proteins that specially bind K63 polyUb chains in whole cell or tissue lysates; 2) validation of direct interactions between K63 polyUb chains and their binding proteins.
The non-cleavable 6xHis-tagged K63 polyUb chains (2-4) are resistant of deubiquitination when incubating with whole cell or tissue lysates, thus allowing maximally capture proteins that specifically bind K63 polyUb chains. After binding, the non-cleavable K63 polyUb chains and the binding proteins can be enriched by Nickel XPure Agarose Resin (included). The bound proteins and the non-cleavable K63 polyUb chains can be eluted from Ni resin using a buffer containing 250 mM imidazole (included) or by incubating with thrombin (not included). Either of the elution method can significantly reduce the amounts of non-specific binding proteins when compared with elution using SDS sample buffer.
Product Name:
K63 polyUb Chain-binding Protein Identification / Validation Kit
>Also Known As:
N/A
>Catalog No.:
J2510
>Size:
1 Kit
>Molecular Weight:
N/A
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
95% based on SDS-PAGE
>Storage:
See the storage condition on the label of each component; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF datasheet
>NCBI RefSeq:
N/A
>Image(s):
No
>Shipping Method:
Dry ice shipping
>References:
N/A SAR405838 18484849
