TNFalpha

TNFalpha (Tumor necrosis factor alpha) is produced by immune and epithelial cells. It plays important roles in regulating inflammation, apoptosis, and immune response. It exerts its function mainly by binding of two cell surface receptors TNFR1 and TNFR2. Treating cells with TNFalpha activates the NFkappaB, MAPK, and death signaling pathways. The recombinant TNFalpha protein consists of the extracellular domain (amino acids 77-233) of full length TNFalpha. It induces apoptosis of various cancer cells at the concentration of 10-100 ng/mL + 1 ug/mL cycloheximide (a protein translation inhibitor). It is generated from GST-TNFalpha (catlog # U1130) by removal of the N-terminal GST tag.

Product Name:
TNFalpha
>Also Known As:
TNF; DIF; TNFA; TNFSF2; TNF-alpha
>Catalog No.:
U1120
>Size:
50 µg
>Molecular Weight:
25.6 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
~ 85% by SDS-PAGE
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet
>NCBI RefSeq:
NM_000594.3
>Image(s):
(Click image to enlarge)