K29/K33 Polyubiquitin Chain Capture Kit
This kit is designed for enrichment of cellular proteins conjugated with lysine 29/33-linked polyubiquitin chains using GST-TRABID (ZNF1). Polyubiquitinated proteins bound on GST-TRABID (ZNF1) can be precipi-tated using glutathione resin and eluted by a buffer containing 10 mM glutathione. Enriched polyubiqui-tinated proteins can be assayed by immunoblotting or mass spectrometry.
Product Name:
K29/K33 Polyubiquitin Chain Capture Kit
>Also Known As:
N/A
>Catalog No.:
J4470
>Size:
1 Kit
>Molecular Weight:
N/A
>Species:
N/A
>Source:
N/A
>Stock:
Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
Enzyme >95% by SDS-PAGE
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF datasheet
>NCBI RefSeq:
N/A
>Image(s):
Images:
(Click image to enlarge)
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A procedure for capturing K29/K33 polyubiquitinated proteins in cell or tissue lysates |
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GST-TRABID(NZF1) pulldown assay 1. 20 ug cell lysates 2. GST pulldown 3. GST-TRABID(NZF1) pulldown |
>Shipping Method:
Dry ice shipping
>References:
N/A TP-0903 18483845
