Unconjugated (Free) Polyubiquitin Chain Capture Kit
The Unconjugated (Free) Polyubiquitin Chain Capture Kity enrich free polyUb chains in cell or tissue extracts within 6 hours. This approach uses the N-terminal ZnF-UBP domain of the deubiquitinating enzyme Usp5 to specifically capture free polyUb chains. The ZnF-UBP domain of Usp5 binds the C-terminal diglycine motif in free Ub chains, it does not bind polyUb chains conjugated on substrate proteins.
Product Name:
Unconjugated (Free) Polyubiquitin Chain Capture Kit
>Also Known As:
N/A
>Catalog No.:
J4420
>Size:
1 Kit
>Molecular Weight:
No
>Species:
No
>Source:
No
>Stock:
Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
Enzyme >95% by SDS-PAGE
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF datasheet, MSDS
>NCBI RefSeq:
N/A
>Image(s):
No
>Shipping Method:
Dry ice shipping
>References:
No GSK461364 18483658/
