Name:
RNase R Protein
Synonyms:
Ribonuclease R、RNase R
Species Name:
null
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
Gene Id:
Molecular Weight:
94 kD
Purity:
＞95% by SDS-PAGE
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
Stability Storage:
Store at -25 ~ -15℃ for 1 years
Buffer System:
50mM Tris-HCl, 200mM NaCl, 1mM DTT, 0.1mM EDTA, 50% (v/v) Glycerol, 0.1% (w/v) Triton X-100 (pH7.5 @25℃)
Quality Statement:
Ribonuclease R (RNase R) is a Mg2+ dependent 3’→5′ exonuclease derived from Escherichia coli. RNase R can digest all linear RNA. However, it cannot digest ring-shaped RNA, lasso structure or double-stranded RNA molecules with 3 ‘end protruding ends less than 7 nucleotides, tRNA and 5SRNA with complex secondary structure. RNase R is commonly used in gene expression and variable shear studies and can digest linear RNA to enrich circular RNA or lasso structured RNA. This product does not contain DNase, other RNA endonuclease and exonuclease activities.
Reference:
[1] Cheng, Z.-F. Purification and Characterization of the Escherichia coli Exoribonuclease RNase R COMPARISON WITH RNase II[J]. Journal of Biological Chemistry, 2002, 277(24):21624. DOI:10.1074/jbc.M202942200. [2] Cheng Z F, Deutscher M P. An Important Role for RNase R in mRNA Decay[J].Molecular Cell, 2005, 17(2):313-318.DOI:10.1016/j.molcel.2004.11.048.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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