Name:
RNase Ⅲ Protein
Synonyms:
Ribonuclease 3,Ribonuclease III,RNase III
Species Name:
null
Label Name:
null
Marker Name:
Unconjugated
Accession:
P0A7Y0
Gene Id:
/
Molecular Weight:
70 kD
Purity:
＞95% by SDS-PAGE
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
/
Stability Storage:
Store at -25 ~ -15℃ for 2 years
Buffer System:
500 mM NaCl、10 mM Tris-HCl、0.5 mM EDTA、1 mM DTT、50% Glycerol\n（pH 8 @ 25°C）
Quality Statement:
The processing of double-stranded(ds) RNA by RNase III family members is an essential step in the maturation and decay of coding and noncoding RNAs, including miRNAs and siRNAs. RNase III family members share a unique fold (RNase III domain) that can dimerize to form a structure that binds dsRNA and cleaves phosphodiesters on each strand, providing the characteristic 2 nt, 3′-overhang product ends. RNase III converts long double-stranded RNA into a heterogeneous mix of short (18–25 bp) interfering RNAs (siRNA).
Reference:
[1] Nicholson AW. Ribonuclease III mechanisms of double-stranded RNA cleavage. Wiley Interdiscip Rev RNA. 2014 Jan-Feb;5(1):31-48. [2] Wu CX, Xu XJ, Zheng K, Liu F, Yang XD, Chen CF, Chen HC, Liu ZF. Characterization of ribonuclease III from Brucella. Gene. 2016 Apr 1;579(2):183-92. [3] Sun, Weimei et al. Catalytic mechanism of Escherichia coli ribonuclease III: kinetic and inhibitor evidence for the involvement of two magnesium ions in RNA phosphodiester hydrolysis. Nucleic Acids Research 33 (2005): 807 – 815.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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