Name:
Pyrophosphatase Protein
Synonyms:
Inorganic pyrophosphatase、PPase
Species Name:
Yeast
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
P00817
Gene Id:
Molecular Weight:
33.5 kD
Purity:
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
Stability Storage:
Store at -25 ~ -15℃for 2 years
Buffer System:
20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50% Glycerol (pH 8.0 @ 25°C)
Quality Statement:
Pyrophosphatase, Inorganic is a yeast derivedinorganic pyrophosphatase (PPase) expressed by Escherichia coli, which is anenzyme that catalyzes the conversion of one molecule of pyrophosphate to twomolecules of phosphate ions, causing the inorganic pyrophosphate hydrolysis toform phosphates. In molecular biology, it can be used to increase theproduction of RNA in vitro transcription reactions.
Reference:
[1] Lahti R , Pitk RantaT , Valve E ,et al. Cloning and characterization of the geneencoding inorganic pyrophosphatase of Escherichia coli K-12.[J].Journal ofBacteriology, 1988, 170(12):5901-7 [2] Satoh T , Watanabe M ,Nogi S I ,et al. Molecular Cloning, Expression, and Site-DirectedMutagenesis of Inorganic Pyrophosphatase from Thermus thermophilus HB8[J]. Journalof Biochemistry, 1998, 124(1):79-88.
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