Name:
Murine RNase Inhibitor Protein
Synonyms:
Ribonuclease inhibitor
Species Name:
Rat
Label Name:
MBP Tag, His Tag
Marker Name:
Unconjugated
Accession:
P29315
Gene Id:
Molecular Weight:
94kDa (Reducing)
Purity:
>95% by SDS-PAGE&HPLC
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
Stability Storage:
Store at -25 ~ -15℃for 2 years
Buffer System:
20 mM HEPES-KOH、50 mM KCl、8 mM DTT、50% Glycerol (pH 7.6 @ 25°C)\n
Quality Statement:
Murine RNase inhibitor is expressed in soluble form inE. coli and capable of inhibiting a wide range of RNases (RNase A, B, C).Murine RNase inhibitor was tested by RT-PCR and RT-qPCR, and was compatiblewith various commercial reverse transcriptase’s and various DNA polymerases.Compared with human RNase inhibitor, murine RNase inhibitor does not containtwo cysteines that are very sensitive to oxidation, so it has higherantioxidant activity and is more suitable for high DTT sensitive experiments (suchas qPCR).
Reference:
[1] Wanhua, Guo, and et al. High levelsoluble production of functional ribonuclease inhibitor in Escherichia coli byfusing it to soluble partners[J]. Protein Expression & Purification,2011.DOI:10.1016/j.pep.2011.01.015[2] Chen C Z, Shapiro R. Site-specificmutagenesis reveals differences in the structural bases for tight binding ofRNase inhibitor to angiogenin and RNase A.[J]. Proceedings of the NationalAcademy of Sciences, 1997, 94(5):p. 1761-1766.DOI:10.1073/pnas.94.5.1761
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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