Name:
Spike Protein
Synonyms:
2019 nCOV Spike S蛋白;S protein; Spike glycoprotein; S glycoprotein; COVID-19
Species Name:
SARS-CoV-2
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
P0DTC2
Gene Id:
Val16-Lys1211(R682S, R685S, K986P&V987P)VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPSRASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKGGGSHHHHHHHH
Molecular Weight:
130-180kDa (Reducing)
Purity:
＞95% by SDS-PAGE
Physical Appearance Name:
Lyophilized Powder
Endotoxin Name:
<1EU/μg
Reconstitution:
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability Storage:
·12months from date of receipt, -20 to -70 °C as supplied. ·1 month, 2 to 8 °C under sterile conditions afterreconstitution. ·Please avoid repeated freeze-thaw cycles.
Buffer System:
PBS, 5% trehalose, pH7.4.
Quality Statement:
Coronaviruses that infect humans belong to theAlpha-coronavirus (including HCoV-229E) and Beta-coronavirus (includingSARS-CoV and SARS-CoV-2) genera. In particular, SARS-CoV-2 is currently a majorthreat to public health worldwide. The spike (S) homotrimers bind to theirreceptors via the receptor-binding domain (RBD), which is a major target toblock viral entry.our results reveal different vaccine strategies for coronaviruses, andS-trimer is better than RBD as a target for vaccine developmentin Alpha-coronavirus Our findings will provide important implicationsfor future development of coronavirus vaccines. Outbreak of coronaviruses,especially SARS-CoV-2, poses a serious threat to global public health.Development of vaccines to prevent the coronaviruses that can infect humans hasalways been a top priority. Coronavirus spike (S) protein is considered as amajor target for vaccine development. Currently, structural studies have shownthat Alpha-coronavirus (HCoV-229E)and Beta-coronavirus (SARS-CoV and SARS-CoV-2) RBDs are in\”lying\” and \”standing\” states in the prefusion S-trimerstructure. Here, we evaluated the ability of S-trimer and RBD to induceneutralizing antibodies among these coronaviruses. Our results showed that theS-trimer and RBD are both candidates for subunit vaccinesin Beta-coronavirus (SARS-CoV and SARS-CoV-2) with a RBD\”standing\” state. However,for Alpha-coronavirus (HCoV-229E) with a RBD \”lying\” state,the S-trimer may be more suitable for subunit vaccines than the RBD. Ourresults will provide novel ideas for the development of vaccines targeting Sprotein in the future.
Reference:
1. Yuejun Shi, Jiale Shi, Limeng Sun, Yubei Tan,Gang Wang, Fenglin Guo, Guangli Hu, Yanan Fu, Zhen F Fu, Shaobo Xiao.Insightinto vaccine development for Alpha-coronaviruses based on structural andimmunological analyses of spike proteins. J Virol. 2021 Mar 10;95(7):e02284-20.doi: 10.1128/JVI.02284-20. Epub 2021 Jan 7.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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