Name:
Recombinant SUMO Protease Protein
Synonyms:
Species Name:
Yeast
Label Name:
No Tag
Marker Name:
Unconjugated
Accession:
Q02724
Gene Id:
Molecular Weight:
27 kDa (Reducing)
Purity:
＞95% by SDS-PAGE and RP-HPLC
Physical Appearance Name:
Liquid
Endotoxin Name:
<1EU/μg
Reconstitution:
Stability Storage:
· 12 months from date of receipt, -20 to -70 °C as supplied. · 6 months, -20 to -70 °C under sterile conditions after reconstitution.· 1 week, 2 to 8 °C under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Buffer System:
20mM PB, pH7.4, 200mM NaCl, 0.1% NP-40, 0.5mM DTT, 50%(v/v) Glycerol
Quality Statement:
Saccharomyces cerevisiae-derived Small ubiquitin-like modifier (SUMO, Smt3) is commonly used as a protein fusion domain to facilitate expression and purification of recombinant proteins, and a Saccharomyces cerevisiae-derived SUMO-specific protease(Ulp1) is then used to remove SUMO tag from these proteins in a ‘scarless’ manner. SUMO Protease cleaves in a highly specific manner, recognizing the tertiary structure of the SUMO tag, rather than an amino acid sequence, and hydrolyzes the peptide bond in the x-Gly-Gly-x sequence after the Gly-Gly bond at the C-terminus of the SUMO tag. The SUMO Protease cleavage proteins over wide ranges of temperature (4℃-30℃), ionic strengths(0-400 mM NaCl) and pH(7.0-9.0), and easily removed from the cleavage reaction by Immobilized Metal Affinity chromatography (IMAC).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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