Ubiquitin Linkage Screening Kit I
Ubiquitin Linkage Screening Kit is designed for two purposes: 1) to identify the ubiquitin lysine residue that is used to link the polyubiquitin chain conjugated on a protein substrate; 2) to determine whether a protein substrate is polyubiquitinated or conjugated with multiple monoubiquitin.
A typical 20 µl reaction contains 100 nM UBE1, 2 µM E2 (not supplied), 50 µM Ub or Ub mutants, 4 mM ATP, 2 µM E3 (not supplied) and 2 µM substrate (no supplied) in the ubiquitination buffer.
Product Name:
Ubiquitin Linkage Screening Kit I
>Also Known As:
N/A
>Catalog No.:
J3200
>Size:
1 Kit
>Molecular Weight:
N/A
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
95% based on SDS-PAGE
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF datasheet, MSDS
>NCBI RefSeq:
N/A
>Image(s):
N/A
>Shipping Method:
Dry ice shipping
>References:
N/A GDC-0068 (dihydrochloride) 18478885
