Sumo3 Conjugation Kit
Sumo3 Conjugation Kit is designed for assaying the conjugation of Sumo3 to protein substrates in vitro. In a protein sumoylation reaction, a Sumo protein is first activated by the Sumo activating enzyme SAE1/SAE2 (E1) in an ATP hydrolysis-dependent manner; activated Sumo is then transferred to the Sumo conjugating enzyme Ube2I (E2) by formation of a thiolester bond with the catalytic cysteine residue of Ube2I; Sumo-charged Ube2I can mediate protein sumoylation in the presence of an appropriate Sumo ligase (E3).
Product Name:
Sumo3 Conjugation Kit
>Also Known As:
N/A
>Catalog No.:
J3130
>Size:
1 Kit
>Molecular Weight:
N/A
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
95% based on SDS-PAGE
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF datasheet, MSDS
>NCBI RefSeq:
N/A
>Image(s):
N/A
>Shipping Method:
Dry ice shipping
>References:
N/A LY2157299 18478813/
