Name:
GP120 Protein
Synonyms:
GP120, GP120-CN54
Species Name:
HIV
Label Name:
No Tag
Marker Name:
Unconjugated
Accession:
P05877
Gene Id:
Lys32-Arg513, with C-terminal 6* HisKLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATQACVPTDPNPQEVELVNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDLRNTTNTNNSTANNNSNSEGTIKGGEMKNCSFNITTSIRDKMQKEYALLYKLDIVSIDNDSTSYRLISCNTSVITQACPKISFEPIPIHYCAPAGFAILKCNDKKFSGKGSCKNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSENFTDNAKTIIVHLNESVQINCTRPNYNKRKRIHIGPGRAFYTTKNIIGTIRQAHCNISRAKWNDTLRQIVSKLKEQFKNKTIVFNQSSGGDPEIVMHSFNCGGEFFYCNTSPLFNSTWNGNNTWNNTTGSNNNITLQCKIKQIINMWQEVGKAMYAPPIEGQIRCSSNITGLLLTRDGGKDTDTNDTEIFRPGGGDMRDNWRSELYKYKVVTIEPLGVAPTKAKRRVVQREKRGGGSHHHHHH
Molecular Weight:
95-118kDa(Reducing)
Purity:
＞95% by SDS-PAGE
Physical Appearance Name:
Lyophilized Powder
Endotoxin Name:
<0.1EU/μg
Reconstitution:
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability Storage:
·12 months from date of receipt, -20 to -70 °C as supplied. ·1 month, 2 to 8 °C under sterile conditions after reconstitution. ·Please avoid repeated freeze-thaw cycles.
Buffer System:
PBS, pH7.4
Quality Statement:
Envelope glycoprotein GP120 (or gp120) is the name of the glycoproteinwhich forms the spikes sticking out of a HIV virus particle. The glycoproteingp120 forms a globular structure, consisting of 25 β strands, five α helicesand ten loops. Each envelope glycoprotein complex is formed by a heterodimerresulting from the non-covalent association of two proteins called gp41 andgp120. Glycoprotein gp41 adopts a transmembrane location, anchored to the viral membrane and behavingas physical support of gp120, the latter being fully located on the externalsurface of the HIV-1 membrane. Both gp41 and gp120 are homotrimeric proteins synthesized from a common single chainpolypeptide precursor gp160, whicheventually is cleaved post-transductionally yielding the mature independent envproteins. The gp120 glycans have been suggested to playan important role in viral transmissionthrough interaction with endogenous lectins able to selectively recognizespecific glycan motifs in HIV-1, such as the C-type lectin DC-SIGN, which isfound on the surfaces of dendritic cells and is thought to aid in the transportof the virus to anatomical sites rich in CD4+T cells. Moreover, theseoligosaccharides are crucial in avoiding an efficient neutralizing antibody response against HIV-1in infected people.
Reference:
1.Kwong P.D.Wyatt R..Robinson J.Sweet R.W.SodroskiJ.Hendrickson W.A. Structure of an HIV gp120 envelope glycoprotein in complexwith the CD4 receptor and a neutralizing human antibody.Nature. 1998; 393(98303379): 648-659 2.Pöhlmann, S.; Baribaud, F.; Lee, B.; Leslie, G.J.; Sánchez, M.D.; Hiebenthal-Millow, K.; Münch,J.;Kirchhoff,F.;Doms,R.W.DC-SIGN.Interactions with Human Immunodeficiency Virus Type 1 and 2 and SimianImmunodeficiency VirusJ. Virol.,2001,75, 4664-4672.(b) Cunningham, A.L.;Harman,A.N.;Donaghy H.DC-SIGN ‘AIDS’ HIV immune evasion and infection.Nat. Immunol.,2007, 8, 556-558.3.A Flores , E Quesada.Entry inhibitors directedtowards glycoprotein gp120: an overview on a promising target for HIV-1therapy.Curr Med Chem. 2013 Feb 1;20(6):751-71.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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