N-terminal TAMRA-Ub
N-terminal TARMA-Ub (also called TARMA-Ub) is a fluorescent Ub, in which TAMRA (5-tetramethylrhodamine) is covalently conjugated on the N-terminus of Ub. All seven lysine residues in TARMA-Ub are available for ubiquitination. This product can be used for determination of protein ubiquitination or E1/E2/E3 enzyme activity using sensitive in-gel fluorescence. TAMRA fluorescence is detected using excitation/emission wavelengths at 550 nm/590 nm, respectively.
Product Name:
N-terminal TAMRA-Ub
>Also Known As:
TAMRA-Ub
>Catalog No.:
E1160
>Size:
100 ug
>Molecular Weight:
8977 Da
>Species:
Human
>Source:
Chemical synthesis
>Stock:
20 mM Tris, pH 7.6 at 4 °C, 150 mM NaC and 10% glycerol
>Concentration:
See tube label
>Quality Assurance:
>95% by FPLC.
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
N/A
>Image(s):
See PDF datasheet.
>Shipping Method:
Dry ice shipping
>References:
(1) El Oualid et al. Angew. Chem. Int. Ed. 2010, 49, 10149.
(2) de Jong et al. ChemBioChem 2012, 13, 2251.
(3) Smit et al. J Biol. Chem 2013, 288, 31728. Palbociclib 18476695
