Name:
T4 DNA Ligase Protein
Synonyms:
DNA Ligase、Polydeoxyribonucleotide synthase [ATP]
Species Name:
null
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
Gene Id:
Molecular Weight:
57 kD
Purity:
＞95% by SDS-PAGE & RP-HPLC
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
Stability Storage:
Store at -25 ~ -15℃ for 1 years
Buffer System:
10 mM Tris-HCl、50 mM KCl、1 mM DTT、0.1 mM EDTA、50% Glycerol\n（pH 7.4 @ 25°C）
Quality Statement:
T4 DNA ligase is a type of DNA ligase. DNA ligase catalyzes the formation of phosphodiester bonds at single-stranded DNA breaks in double-stranded DNA in vivo. DNA ligase has important biological functions in organisms. In DNA repair and recombination, DNA ligase plays a role in connecting gaps. In the process of DNA replication, the synthesis of the lagging strand is discontinuous, and DNA ligase connects the discontinuous DNA strand into a continuous DNA strand. It does not contain DNA endonuclease, exonuclease and phosphatase, and does not contain RNA enzyme.
Reference:
[1] Williamson A, Pedersen H. Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida. Protein Expr Purif. 2014 May; 97:29-36. [2] Liu X, Huang A, Luo D, Liu H, Han H, Xu Y, Liang P. Use of adenylate kinase as a solubility tag for high level expression of T4 DNA ligase in Escherichia coli. Protein Expr Purif. 2015 May;109:79-84. doi: 10.1016/j.pep.2015.02.010. Epub 2015 Feb 17. PMID: 25700573.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
MCP-2/CCL8 ProteinStorage & Stability
BNP ProteinMolecular Weight
Popular categories:
CD200
SARS-CoV-2 3C-like Protease
