Name:
RSPO1 Protein
Synonyms:
R-spondin1, Cristin-3, mCristin-3
Species Name:
Mouse
Label Name:
His Tag
Marker Name:
null
Accession:
Q9Z132
Gene Id:
Ser21-Gln265SRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCQEALYLHKGRCYPACPEGSTAANSTMECGSPAQCEMSEWSPWGPCSKKRKLCGFRKGSEERTRRVLHAPGGDHTTCSDTKETRKCTVRRTPCPEGQKRRKGGQGRRENANRHPARKNSKEPRSNSRRHKGQQQPQPGTTGPLTSVGPTWAQGGGSHHHHHHHH
Molecular Weight:
27-30kDa (Reducing)
Purity:
＞95% by SDS-PAGE&RP-HPLC
Physical Appearance Name:
Lyophilized Powder
Endotoxin Name:
<0.1EU/μg
Reconstitution:
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability Storage:
·12 months from date of receipt, -20 to -70 °C as supplied. ·1 month, 2 to 8 °C under sterile conditions after reconstitution. ·Please avoid repeated freeze-thaw cycles.
Buffer System:
PBS, 5% trehalose, PH7.4
Quality Statement:
R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27-30 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members. RSPO1 is a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids.
Reference:
1.Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700. 2.Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247. 3.Wei, Q. et al. (2007) J. Biol. Chem. 282:15903. 4.Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51. 5.Parma, P. et al. (2006) Nat. Genet. 38:1304.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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