Name:
HRV 3C Protease Protein
Synonyms:
Protease 3C、PreScission Protease
Species Name:
null
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
Gene Id:
/
Molecular Weight:
21 kD (Reducing)
Purity:
>95% by SDS-PAGE
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
/
Stability Storage:
Store at -25 ~ -15℃ for 2 years
Buffer System:
50mM Tris-HCl,150mM NaCl,10mM EDTA,1mM DTT,50%(v/v) glycerol,(pH 8.0 @ 25℃)
Quality Statement:
Recombinant human rhinovirus(HRV)3C protease is widely used in the purification and productions of proteins due to its good stability,high specificity,and enzymatic activity.3C protease is one of the non—structural proteins of HRV,and plays important roles in the life cycle of HRV.This protease specifically recognizes the sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, and cleaves the peptide bond between Gln and Gly.
Reference:
[1] Knott J A , Orr D C , Montgomery D S ,et al.The expression and purification of human rhinovirus protease 3C.[J].European Journal of Biochemistry, 1989, 182(3):547-55.DOI:10.1111/j.1432-1033.1989.tb14862.x.[2] Xu H , Wang Q , Zhang Z ,et al.A simplified method to remove fusion tags from a xylanase of Bacillus sp. HBP8 with HRV 3C protease[J].Enzyme and Microbial Technology, 2019, 123:15-20.DOI:10.1016/j.enzmictec.2019.01.004.
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