Name:
Endo H Protein
Synonyms:
Endo-beta-N-acetylglucosaminidase H、Endoglycosidase H、Endo H
Species Name:
Streptomyces picatus
Label Name:
null
Marker Name:
Unconjugated
Accession:
Gene Id:
/
Molecular Weight:
72kD (Reducing)
Purity:
＞95% by SDS-PAGE and HPLC
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
/
Stability Storage:
Store at -25 ~ -15℃ for 2 years
Buffer System:
20 mM Tris-HCl、50 mM NaCl、5 mM EDTA（pH 7.5 @ 25°C）
Quality Statement:
Endo H is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E.coli. It cleaves the chitobiose core of high-mannose oligosaccharides and a limited number of hybrid oligosaccharides from asparagine-linked glycoproteins, but not complex, oligosaccharides from glycoproteins.
Reference:
[1] Wang F , Wang X , Yu X ,et al. High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins[J].Plos One, 2015, 10.[2] Maley F, Trimble R B, Tarentino A L,et al. Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases.[J]. Analytical Biochemistry,1989,180(2):195-204.DOI:10.1016/0003-2697(89)90115-2.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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