Name:
PNGase F Protein
Synonyms:
Peptide-N(4)-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F, Peptide N-Glycosidase F, PNGase F
Species Name:
Elizabethkingia miricola
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
P21163
Gene Id:
Molecular Weight:
36kDa (Reducing)
Purity:
＞95% by SDS-PAGE and HPLC
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
Stability Storage:
Store at -25 ~ -15℃for 2 years
Buffer System:
20 mM Tris-HCl、50 mM NaCl、5 mM EDTA（pH 7.5 @ 25°C）
Quality Statement:
Peptide:N-glycosidase F (PNGase F) is an asparagine amidase produced by Flavobacteriummeningosept-icum that serves as a useful tool in the research on proteinN-glycosylation. The cleavage site of PNGase F is the amide bond betweenN-acetylglucosamine (GlcNAc) and aspartate residues on the medial side of theglycoprotein, and converts aspartyl to aspartic acid on the enzymolysisprotein. This productoverexpressed in E.coliand used for complete deglycosylation of antibodies and their associatedproteins
Reference:
[1] FrankMaley and Robert B. Trimble and Anthony L. Tarentino and Thomas H. Plummer Jr. Characterizationof glycoproteins and their associated oligosaccharides through the use ofendoglycosidases[J]. Analytical Biochemistry, 1989.. [2] B, Ling Hua A, et al. Highly efficient productionof peptides: N -glycosidase F for N -glycomics analysis[J]. Protein Expressionand Purification, 2014, 97(5):17-22.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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