PNGase F Protein | Adenosylmethionine

Name:
PNGase F Protein

Synonyms:
Peptide-N(4)-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F, Peptide N-Glycosidase F, PNGase F

Species Name:
Elizabethkingia miricola

Label Name:
His Tag

Marker Name:
Unconjugated

Accession:
P21163

Gene Id:

Molecular Weight:
36kDa (Reducing)

Purity:
＞95% by SDS-PAGE and HPLC

Physical Appearance Name:
Liquid

Endotoxin Name:
null

Reconstitution:

Stability Storage:
Store at -25 ~ -15℃for 2 years

Buffer System:
20 mM Tris-HCl、50 mM NaCl、5 mM EDTA（pH 7.5 @ 25°C）

Quality Statement:
Peptide:N-glycosidase F (PNGase F) is an asparagine amidase produced by Flavobacteriummeningosept-icum that serves as a useful tool in the research on proteinN-glycosylation. The cleavage site of PNGase F is the amide bond betweenN-acetylglucosamine (GlcNAc) and aspartate residues on the medial side of theglycoprotein, and converts aspartyl to aspartic acid on the enzymolysisprotein. This productoverexpressed in E.coliand used for complete deglycosylation of antibodies and their associatedproteins

Reference:
[1] FrankMaley and Robert B. Trimble and Anthony L. Tarentino and Thomas H. Plummer Jr. Characterizationof glycoproteins and their associated oligosaccharides through the use ofendoglycosidases[J]. Analytical Biochemistry, 1989.. [2] B, Ling Hua A, et al. Highly efficient productionof peptides: N -glycosidase F for N -glycomics analysis[J]. Protein Expressionand Purification, 2014, 97(5):17-22.

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Author: Adenosylmethionine- apoptosisinducer

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