Name:
Endo H Protein
Synonyms:
Endo-beta-N-acetylglucosaminidase H, Endoglycosidase H, Endo H
Species Name:
Streptomyces picatus
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
P04067
Gene Id:
Molecular Weight:
30kD (Reducing)
Purity:
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
Stability Storage:
Store at -25 ~ -15℃for 2 years
Buffer System:
20 mM Tris-HCl、50 mM NaCl、5 mM EDTA(pH 7.5 @ 25°C)
Quality Statement:
Endo H is a recombinant glycosidase cloned fromStreptomyces plicatus and overexpressed in E.coli. It cleaves thechitobiose core of high-mannose oligosaccharides and a limited number of hybridoligosaccharides from asparagine-linked glycoproteins, but not complex,oligosaccharides from glycoproteins.
Reference:
[1] Wang F , Wang X , Yu X ,et al. High-LevelExpression of Endo-β-N-AcetylglucosaminidaseH from Streptomyces plicatus in Pichia pastoris and Its Application for theDeglycosylation of Glycoproteins[J].Plos One, 2015, 10. [2] Maley F, Trimble R B,Tarentino A L,et al. Characterization of glycoproteins and their associatedoligosaccharides through the use of endoglycosidases.[J]. Analytical Biochemistry,1989,180(2):195-204.DOI:10.1016/0003-2697(89)90115-2.
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