Name:
DNase Ⅰ Protein
Synonyms:
DNASE,Deoxyribonuclease-1
Species Name:
Bovine Pancreatic
Label Name:
MBP Tag, His Tag
Marker Name:
Unconjugated
Accession:
P00639
Gene Id:
Molecular Weight:
72kDa (Reducing)
Purity:
>95% by SDS-PAGE&HPLC
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
Stability Storage:
Store at -25 ~ -15℃for 2 years
Buffer System:
10 mM Tris-HCl, 2 mM CaCl2 ,50% Glycerol，(pH 7.6, 25°C)
Quality Statement:
DNase I(Deoxyribonuclease I), can digest single or double-stranded DNA to produce monodeoxynucleotides or single or double-stranded oligo deoxynucleotides, itsoptimal working pH range is 7-8. DNase I activity is dependent on Ca2+and can be activated by other bivalent metal ions such as Mg2+, Mn2+,Zn2+, etc. In the presence of Mg2+, the enzyme canrandomly recognize and cut any site on any strand of DNA. In the presence of Mn2+,two strands of DNA can be cut at the same site to form sticky ends with flatends or 1-2 nucleotides protruding.
Reference:
[1] Vanecko S, Laskowski M. Studies of theSpecificity of Deoxyribonuclease I[J]. Journal of Biological Chemistry, 1961,236(236):3312-6.[2] Kienzle N, Young D, Zehntner S, et al. DNaseItreatment is a prerequisite for the amplification of cDNA from episomal-basedgenes[J]. Biotechniques, 1996, 20(4):612-6. [3] Michael,R, Green, etal. Human β-globin pre-mRNA synthesized in vitro is accurately spliced inxenopus oocyte nuclei[J].Cell, 1983, 32(3):681-694.
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