Name:
BsaⅠ Protein
Synonyms:
BsaⅠ 限制性内切酶
Species Name:
Bacillus stearothermophilus
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
Q6SPF4
Gene Id:
Molecular Weight:
64.9kDa (Reducing)
Purity:
>95% by SDS-PAGE
Physical Appearance Name:
Liquid
Endotoxin Name:
null
Reconstitution:
Stability Storage:
Store at -25 ~ -15℃for 2 years
Buffer System:
10 mM Tris-HCl、300 mM NaCl、1 mM DTT、0.1 mM EDTA、500 µg/ml BSA、50% Glycerol （pH 7.4 @ 25°C）
Quality Statement:
BsaIis a Type IIs restriction enzyme that can recognize non-palindromic sequencesand cut outside of the recognition sequence. It is commonly used for GoldenGate assembly and enzymatic cleavage of plasmids to prepare linear DNAfragments with poly (A/T/G/C) endings and obtain specific sticky ends.Recognitionsite:5′-GGTCTC(N)1↓-3′ 3′-CCAGAG(N)5↑-5′
Reference:
[1]Zhu Z, Samuelson J C, Zhou J, et al.Engineering Strand-specific DNA NickingEnzymes from the Type IIS Restriction Endonucleases BsaI, BsmBI, andBsmAI[J].Journal of Molecular Biology, 2004,337(3):573-583.DOI:10.1016/j.jmb.2004.02.003. [2] Lee J H, Won H J, Oh E S,etal. Golden GateCloning-Compatible DNA Replicon/2A-Mediated Polycistronic Vectors forPlants[J]. Frontiers in Plant Science, 2020, 11.DOI:10.3389/fpls.2020.559365.
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