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Name:
Luciferase Ⅱ Protein

Synonyms:

Species Name:
Photinus pyralis

Label Name:
His Tag

Marker Name:
Unconjugated

Accession:
P08659

Gene Id:
N.A

Molecular Weight:
61.7kDa

Purity:
>95% by SDS-PAGE

Physical Appearance Name:
Liquid

Endotoxin Name:
null

Reconstitution:
N.A

Stability Storage:
12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.

Buffer System:
20 mM Tris (pH7.4), 1 mM EDTA, 1 mM DTT, 50% glycerol

Quality Statement:
Luciferase is a general term for enzymes that produce biofluorescence in nature. Luciferase can catalyze the oxidation of luciferin to oxyluciferin. In the process of luciferin oxidation, biofluorescence is emitted. The biofluorescence released during the oxidation of luciferin can then be measured by a fluorometer. In our work, the luciferase came from the firefly Photinus pyralis (Ppy) catalyzes a two-step reaction that results in the oxidation of D-luciferin accompanied by emission of yellow−green light with a peak at 560 nm. However, some biological applications are limited by the low stability of the luciferase, so we combined amino acid mutations to enhance the enzyme’s thermostability and was eventually named Luciferase Ⅱ.

Reference:
1.Mcelroy H H S D .THE COLORS OF FIREFLY BIOLUMINESCENCE: ENZYME CONFIGURATION AND SPECIES SPECIFICITY[J].Proceedings of the National Academy of Sciences of the United States of America, 1964, 52(1):75-81.DOI:10.1073/pnas.52.1.75.2.Conti E , Franks N P , Brick P .Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes[J].Structure, 1996, 4(3):287.DOI:10.1016/S0969-2126(96)00033-0.

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Author: Adenosylmethionine- apoptosisinducer