Name:
NELFE Protein
Synonyms:
RD; RDP; RDBP; D6S45; NELF-E
Species Name:
Human
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
P18615
Gene Id:
Met1-Phe380 with N terminal 8*His HHHHHHHHMLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDRDRERDRDRDRDRDRERDRDRDRDRDRDRERDRDRERDRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF
Molecular Weight:
44kDa
Purity:
Physical Appearance Name:
Lyophilized Powder
Endotoxin Name:
<0.1EU/μg
Reconstitution:
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability Storage:
12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.
Buffer System:
20mM Tris, 500mM NaCl, 2mM DTT, pH8.0
Quality Statement:
Negative elongation factor E (NELFE) is part of a complex termed NELF which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). Further mechanistic studies revealed that NELFE activates the Wnt/β‑catenin signaling pathway by decreasing the stabilization of NDRG2 mRNA in pancreatic cancer. oncogenic activation of NELFE promotes hepatocellular carcinoma (HCC) cell proliferation and predicts the strong metastatic potential of HCC cells.
Reference:
1.Yu S, Li L, Cai H, He B, Gao Y, Li Y. Overexpression of NELFE contributes to gastric cancer progression via Wnt/β-catenin signaling-mediated activation of CSNK2B expression. J Exp Clin Cancer Res. 2021 Feb 1;40(1):54. doi: 10.1186/s13046-021-01848-3. PMID: 33526068; PMCID: PMC7851912.2.Han L, Zan Y, Huang C, Zhang S. NELFE promoted pancreatic cancer metastasis and the epithelial?to?mesenchymal transition by decreasing the stabilization of NDRG2 mRNA. Int J Oncol. 2019 Dec;55(6):1313-1323. doi: 10.3892/ijo.2019.4890. Epub 2019 Oct 2. PMID: 31638184; PMCID: PMC6831195.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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