Name:
Carbonic Anhydrase IX/CA9 Protein
Synonyms:
Carbonate dehydratase IX, Carbonic Anhydrase IX (CA-IX; CAIX), Membrane antigen MN, P54/58N
Species Name:
Human
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
Q16790
Gene Id:
Gln38-Asp414, with C-terminal 8*HisQRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNSCLAAGDGGGSHHHHHHHH
Molecular Weight:
51-54kDa
Purity:
＞95% by SDS-PAGE
Physical Appearance Name:
Lyophilized Powder
Endotoxin Name:
<0.1EU/μg
Reconstitution:
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability Storage:
12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.
Buffer System:
PBS, pH7.4
Quality Statement:
The CAIX works as a dimeric molecule composed of two monomeric proteins liked with a disulfide bond between cysteine residues of the two CAIX monomers, while the structure of CAXII is stabilized by 19 hydrogen-bonded interactions in the dimer interface. Carbonic Anhydrases IX and CAXII (CAIX/CAXII) are transmembrane zinc metalloproteins that catalyze a very basic but crucial physiological reaction: the conversion of carbon dioxide into bicarbonate with a release of the proton. CAIX functions with bicarbonate transporters (including electroneutral bicarbonate transporter NBCn1 (SLC4A7)) to bring in HCO3- produced in a CAIX-mediated hydration reaction of CO2, and amino acid transporters (including the large neutral amino acid transporter 1 (LAT1) and CD98 heavy chain (CD98hc)) to transport in essential amino acids and glutamine (via interaction with glutamine transporter SLC1A5 and sodium-coupled neutral amino acid transporter 2 (SNAT2) facilitating the uptake of amino acid by SLC1A5), which cancer cells employ as an alternative metabolic energy source and biosynthetic precursor. CAIX also creates unique interactions with collagen- and laminin-binding integrins at the leading edge of migrating cells.
Reference:
1.\tSupuran CT. Carbonic Anhydrases: novel therapeutic applications for inhibitors and activators. Nat Rev Drug Discov 2008;7:168-81. 2.\tMcDonald PC, Swayampakula M, Dedhar S.. Coordinated regulation of metabolic transporters and migration/invasion by Carbonic Anhydrase IX. Metabolites 2018;8:E20.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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