Name:
Carbonic Anhydrase IX/CA9 Protein
Synonyms:
EC:4.2.1.1，Renal cell carcinoma-associated antigen G250，P54/58N，Carbonic Anhydrase 9，RCC-Associated Protein G250，Carbonate Dehydratase IX，Carbonic Dehydratase，CA-IX，CAIX，PMW1，MN，EC 4.2.1.1，G250，Membrane antigen MN，RCC-associated antigen G250
Species Name:
Mouse
Label Name:
His Tag
Marker Name:
Unconjugated
Accession:
Q8VHB5
Gene Id:
Gln32-Asp390, with C-terminal 8*HisQPQGLSGMQGEPSLGDSSSGEDELGVDVLPSEEDAPEEADPPDGEDPPEVNSEDRMEESLGLEDLSTPEAPEHSQGSHGDEKGGGHSHWSYGGTLLWPQVSPACAGRFQSPVDIRLERTAFCRTLQPLELLGYELQPLPELSLSNNGHTVQLTLPPGLKMALGPGQEYRALQLHLHWGTSDHPGSEHTVNGHRFPAEIHVVHLSTAFSELHEALGRPGGLAVLAAFLQESPEENSAYEQLLSHLEEISEEGSKIEIPGLDVSALLPSDLSRYYRYEGSLTTPPCSQGVIWTVFNETVKLSAKQLHTLSVSLWGPRDSRLQLNFRATQPLNGRTIEASFPAAEDSSPEPVHVNSCFTAGDGGGSHHHHHHHH
Molecular Weight:
43-55kDa (Reducing)
Purity:
＞95% by SDS-PAGE
Physical Appearance Name:
Lyophilized Powder
Endotoxin Name:
<0.1EU/μg
Reconstitution:
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability Storage:
·12 months from date of receipt, -20 to -70 °C as supplied. ·1 month, 2 to 8 °C under sterile conditions after reconstitution. ·Please avoid repeated freeze-thaw cycles.
Buffer System:
PBS, pH7.4
Quality Statement:
The CAIX works as adimeric molecule composed of two monomeric proteins liked with a disulfide bondbetween cysteine residues of the two CAIX monomers, while the structure ofCAXII is stabilized by 19 hydrogen-bonded interactions in the dimer interface. Carbonicanhydrases IX and CAXII (CAIX/CAXII) are transmembrane zinc metalloproteinsthat catalyze a very basic but crucial physiological reaction: the conversion ofcarbon dioxide into bicarbonate with a release of the proton.CAIX functionswith bicarbonate transporters (including electroneutral bicarbonate transporterNBCn1 (SLC4A7)) to bring in HCO3- produced in a CAIX-mediated hydrationreaction of CO2, and amino acid transporters (including the large neutral aminoacid transporter 1 (LAT1) and CD98 heavy chain (CD98hc)) to transport inessential amino acids and glutamine (via interaction with glutamine transporterSLC1A5 and sodium-coupled neutral amino acid transporter 2 (SNAT2) facilitatingthe uptake of aminoacid by SLC1A5), which cancer cells employ as an alternativemetabolic energy source and biosynthetic precursor. CAIX also creates uniqueinteractions with collagen- and laminin-binding integrins at the leading edgeof migrating cells.
Reference:
1.Supuran CT. Carbonic Anhydrases: noveltherapeutic applications for inhibitors and activators. Nat Rev Drug Discov2008;7:168–81.2.McDonald PC, Swayampakula M, Dedhar S..Coordinated regulation of metabolic transporters and migration/invasion byCarbonic Anhydrase IX. Metabolites 2018;8:E20.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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