Name:
Biotin Labeling for Avitag Protein Protein
Synonyms:
生物素标记试剂盒，生物素标记，标记， Bifunctional ligase/repressor, BirA, Biotin-protein ligase
Species Name:
null
Label Name:
No Tag
Marker Name:
Unconjugated
Accession:
P06709
Gene Id:
Met1-Lys321MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK.
Molecular Weight:
35kDa (Reducing)
Purity:
＞95% by SDS-PAGE and HPLC
Physical Appearance Name:
Liquid
Endotoxin Name:
<0.5EU/μg
Reconstitution:
Stability Storage:
12 months from date of receipt, -20℃ as supplied. Store BirA and BirA Buffer at -20℃.Avoid repeated freezing and thawing cycles.
Buffer System:
20mM Bicine, 50% Glycerol, pH8.3
Quality Statement:
BirA, the most characterized biotin-acetyl-CoA-carboxylase ligase, has been successfully applied to biotin labeling with a wide range of protein substrates that fused an acceptor peptide (AP, also known as Avitag) in vivo and in vitro. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5′-adenylate (BirA-bio-5′-AMP or holoBirA) complex. BirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase. Due to its extremely high strength, the interaction between biotin and streptavidin has been exploited for biotechnological applications, like in vivo imaging, cell sorting, et al.
Reference:
1.Howarth M, Ting AY. Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin. Nat Protoc. 2008;3(3):534-45. doi: 10.1038/nprot.2008.20. PMID: 18323822; PMCID: PMC2671200.2.Fairhead M, Howarth M. Site-specific biotinylation of purified proteins using BirA. Methods Mol Biol. 2015;1266:171-84. doi: 10.1007/978-1-4939-2272-7_12. PMID: 25560075; PMCID: PMC4304673.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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