Ve automobiles in for the target the target cells. According to thecould confirm liposomes surroundingblue flu- have been blue fluorescence (nuclei staining), fluorescent liposomes surrounding cellsliposomes autos for cells. Based on the red we red fluorescent that DiI-labeled in cells in blue fluorescence into we could we could confirm that DiI-labeled have been effectively successfully(nuclei staining), cells. orescence delivered(nuclei staining),confirm that DiI-labeled liposomesliposomes were2.4. The Cell Uptake of DiI-Labeled Liposomes in 7F2 Osteoblasts 2.four. The Cell Uptake of DiI-Labeled Liposomes in 7F2 Osteoblasts successfully delivered into cells. delivered into cells.Figure 4. In vitro uptake of DiI-labeled liposomes in 7F2 osteoblasts measured by fluorescent microscopy. The cells had been cultured with DiI-labeled Figure four.TOPS Protocol four. In vitro uptake of DiI-labeledliposomes liposomes osteoblasts measured by fluorescent mi7F2 for 4 h. Thereafter, red fluorescence Figure In vitro uptakeand DiI-labeled liposomes innuclei staining) measured by fluorescent mi(DiI-labeled liposomes) of blue fluorescence (DAPIin 7F2 osteoblasts were evaluated by fluoresfor croscopy. The cellsequipped using a CCD system (00 liposomes for for 4Thereafter, red fluorescence with DiI-labeled liposomes 4 h.2,6-Dihydroxybenzoic acid In Vivo h. = 200 nm). croscopy. The cellswere cultured with DiI-labeled magnification, scale bar Thereafter, red fluorescence cent microscopy had been cultured (DiI-labeled liposomes) and blue fluorescence (DAPI for nuclei staining) were evaluated by fluorescent (DiI-labeled liposomes) and blue fluorescence (DAPI for nuclei staining) have been evaluated by fluorescent microscopy equipped using a CCD system (00 magnification, scale bar = 200 nm). microscopy equipped using a CCD program (00 magnification, scale bar = 200 nm).two.five. The Impact of Astaxanthin and Asta-Loaded Liposomes on Cell Viability of Raw264.7 Mouse Macrophages As shown in Figure 5A, the viability of Raw264.7 mouse macrophages was monitored soon after 24 h incubation with distinctive concentrations of astaxanthin extract.PMID:24140575 Simply because astaxanthin extract could be dissolved in ethanol, ethanol was used as a manage group. The2.five. The Effect of Astaxanthin and Asta-Loaded Liposomes on Cell Viability of Raw264.7 Mouse MacrophagesPharmaceuticals 2022, 15,As shown in Figure 5A, the viability of Raw264.7 mouse macrophages was monitored five of 16 following 24 h incubation with distinctive concentrations of astaxanthin extract. Mainly because astaxanthin extract could be dissolved in ethanol, ethanol was applied as a handle group. The outcome presented that cell viability was decreased inside a dose-dependent manner for the cells treated outcome astaxanthin extract,viability was lowered within a dose-dependent manner forthe cells with presented that cell specifically at high doses (0.51 and 1.02 g/mL). When the cells treated with astaxanthin extract, specifically at higher doses (0.51 and 1.02 /mL). When had been exposed to 0.51 and 1.02 g/mL concentrations of astaxanthin extract, there had been the cells had been exposed reductions in cell viability. Subsequent, we measured cell extract, there about 74 and 95 to 0.51 and 1.02 /mL concentrations of astaxanthin viability for have been aboutmouse macrophages treated cell viability. Next, we measured cell viability for Raw264.7 74 and 95 reductions in with asta-loaded liposomes. As shown in Figure Raw264.7 doses of asta-loaded liposomes (0.51 and 1.02 g/mL) canAs showntheFigure 5B, 5B, high mouse macrophages treated with asta-loaded liposomes. increase.
