Ged pictures (E,F).J Comp Neurol. Author manuscript; obtainable in
Ged images (E,F).J Comp Neurol. Author manuscript; obtainable in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 5.CLSM views of DNA Methyltransferase Gene ID immunofluorescence for VGLUT1 (A) or ALK5 Species VGLUT2 (B) in fields with fluorescent PHAL labeling of thalamostriatal axons and terminals (C,D). Note that thalamostriatal terminals in (C) do not immunolabel for VGLUT1 but those thalamostriatal terminals in (D) do immunolabel for VGLUT2. This could be seen extra clearly in the merged pictures (E,F).J Comp Neurol. Author manuscript; obtainable in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 6.Detail of CLSM photos shown in Figures 4 and five. Pictures in (A,C,E) present magnified views in the reduce left from pictures Fig. 4A,C,E, respectively. Similarly, pictures (B,D,F) present magnified views from the upper left from pictures Fig. 5B,D,F, respectively. These magnified views make it additional achievable to resolve individual terminals, and thereby confirm: 1) PHAL-labeled corticostriatal varicosities that are evident as such by their thickness (arrows) are characteristically immunolabeled for VGLUT1 (A,C,E); and two) PHAL-labeledJ Comp Neurol. Author manuscript; obtainable in PMC 2014 August 25.Lei et al.Pagethalamostriatal varicosities that happen to be evident as such by their thickness (arrows) are characteristically immunolabeled for VGLUT2 (B,D,F).NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; available in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 7.EM pictures of VGLUT2 immunolabeled synaptic terminals in rat striatum ending on spines (A ) or dendrites (E,F). Spines (Sp) were recognizable by their tiny size, the presence of spine apparatus (SA), and the absence of mitochondria (M) and microtubules (Mt), while dendrites (De) had been recognizable by their larger size, the presence of mitochondria and microtubules, and also the absence of spine apparatus. All VGLUT2 synaptic terminals formed asymmetric synaptic contacts, as recognizable by the thick postsynapticJ Comp Neurol. Author manuscript; accessible in PMC 2014 August 25.Lei et al.Pagedensity (PSD). Within the case of some synaptic contacts, the PSD was perforated (asterisks in C,D). All pictures are at the identical magnification shown in (F).NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; out there in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 8.EM photos of VGLUT1 immunolabeled synaptic terminals in rat striatum ending on spines (A ). Spines (Sp) were recognizable by their little size, the presence of spine apparatus (SA), as well as the absence of mitochondria and microtubules. All VGLUT1 synaptic terminals formed asymmetric synaptic contacts, as recognizable by the thick postsynaptic density (PSD). Inside the case of some synaptic contacts, the PSD was perforated (asterisks in C,D). All photos are at the exact same magnification shown in (B).J Comp Neurol. Author manuscript; accessible in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; readily available in PMC 2014 August 25.Figure 9.Size frequency distributions for axospinous (AS) and axodendritic (AD) VGLUT1 and VGLUT2 terminals in rat stria-tum, scaled to t.
