Y expressed FLAG-tagged human RCAN1.1S protein ( 30 kDa; for additional particulars, see Oh et al., 2005; Hoeffer et al., 2007). Reduced blot is stained with a FLAG antibody, confirming expression of transgene. Nse-Cre driver line, CamkII -Cre (T-29) driver line. GAPDH, loading manage. Image is representative of 3 independent blots. WT represents the WT manage for each breeding cross. F, Mice overexpressing human RCAN1 early in improvement (Nse-RCAN1Tg1a) display decreased EPM open-arm time compared with WT littermates. No distinction in open-arm time was observed driving an alternative RCAN1 construct (RCAN1Tg1) with the same Nse-Cre driver. No difference in open-arm time was also observed with postdevelopmental RCAN1 overexpression (CamkII -Cre) of either RCAN1 construct. WT-Tg1a (Nse) denotes manage HDAC4 Inhibitor list RCAN1Tg1a-only (no Cre) “WT” littermates from RCAN1Tg1a Nse-Cre lines. ERK5 Inhibitor web WT-Tg1 (Nse) denotes handle RCAN1Tg1-only (no Cre) “WT” littermates from RCAN1Tg1 Nse-Cre lines. WT-Tg1 (CamkII ) denotes control RCAN1Tg1a-only (no Cre) “WT” littermates from RCAN1Tg1a CamkII -Cre lines. WT-Tg1 (CamkII ) denotes control RCAN1Tg1-only (no Cre) “WT” littermates from RCAN1Tg1 CamkII -Cre lines. N 36 Nse-RCAN1Tg1a, 40 WT-Tg1a (Nse), 7 Nse-RCAN1Tg1, 11 WT-Tg1 (Nse), 32 CamkII -RCAN1Tg1a, 38 WT-Tg1a (CamkII ), 11 CamkII -RCAN1Tg1, 17 WT-Tg1 (CamkII ). For some datasets not usually distributed, Mann hitney evaluation was utilised. p 0.05, or p 0.01, p 0.001.Hoeffer, Wong et al. ?RCAN1 Modulates Anxiety and Responses to SSRIsJ. Neurosci., October 23, 2013 ?33(43):16930 ?6944 ?Table 1. OFA activity of mice that overexpress human RCAN1a Time/zone (s) Distance/zone (cm) Genotype Nse-RCAN1 Mean SEM WT-Tg1a (Nse) Imply SEM p worth Nse-RCAN1Tg1 Mean SEM WT-Tg1 (Nse) Mean SEM p value CamkII -RCAN1Tg1a Imply SEM WT-Tg1a (CamkII ) Imply SEM p worth CamkII -RCAN1Tg1 Imply SEM WT-Tg1 (CamkII ) Imply SEM p valueTg1aAmbulatory (s) Periphery Center Total 62.1 four.three 20.eight 2.four 82.9 four.Resting (s) Periphery Center Total 580.1 13.1 69.9 ten.three 650 six.Vertical (s) Periphery Center Total 92 six.7 7.6 1.7 99.six 7.Stereotypic (s) Periphery Center Total 125.3 2.six 25.three 3.three 150.six three.Periphery Center Periphery Center Total 767.6 15.five 132.four 15.five 1576.two 73.4 513.9 55.6 2090.1 81.779.five 120.5 1596.3 510.6 2106.9 61.7 ten.eight 10.eight 99.7 60.six 128.four 4.9 0.542 0.542 0.87 0.968 0.911 0.952 718.5 35.8 181.5 35.8 1633.3 92.1 572.3 78.two 2205.six 140.9 69.eight 9.20.8 82.5 594.9 two.8 six.2 12.three 0.995 0.956 0.417 19.4 3.1 89.two 11.1 549.five 29.63 658 98.four 6.8 9.5 8.3 0.591 0.475 0.545 101.7 27.two 651.two 16.1 63.7 9.six.3 104.7 122.eight 22.four 145.2 1.2 9.1 3.two 2.1 3.9 0.533 0.669 0.538 0.476 0.284 five.5 2.5 3.72 0.7 0.495 8.9 2.4 7 1.four 0.496 1.3 0.6 1.1 0.7 0.835 69.two ten.3 114.four four.8 29.eight 5.three 144.two 6.732.1 167.9 1551.9 623.0 2174.9 63.0 37.2 37.2 74.7 88.6 105.3 9.2 0.804 0.804 0.502 0.679 0.864 0.622 717.eight 32 746.2 17.9 0.74 799.five 13.two 822.1 ten.five 0.191 182.two 32 1802.1 97.1 677.8 75.two 2479.9 128.six 73.128.two 91.3 536.0 106.five 642.five 44.eight 5.four ten.7 27.1 29.five 14.0 eight.1 0.184 0.894 0.737 0.905 0.686 0.139 27.4 3.1 100.4 6.3 536.9 18.4 85 17.2 621.9 9 77.four 7.48.5 115.4 35.8 151.2 8.6 7.0 7.1 three.0 0.131 0.904 0.517 0.333 86.3 eight.7 131.9 four.1 31.6 3.9 163.five 3.153.8 1873.five 706.eight 2580.3 75.1 17.9 89.5 61.7 120.3 four.7 0.74 0.59 0.765 0.57 0.77 one hundred.five 13.two 1736.5 123.6 357.4 43.1 2093.9 137.6 70 6.30 105 546.7 two.8 6.2 12.six 0.535 0.604 0.659 13.three 1.8 83.3 7 595 14.72.7 619.four 76.four eight.two 9 7.2 0.512 0.844 0.92 50.7 7.7 645.7 11 50.7 7.83.four 128.7 29.7 158.three eight.1 two.6 2.7 three.three 0.8.
