iersin.orgAugust 2021 | Volume 12 | ArticleTorres-Silva et al.De novo Transcriptome of M. glaucescens Shoot OrganogenesisFIGURE 6 | Networks of differentially expressed genes. The networks had been created employing GO categories in BiNGO for M. glaucescens explants prior to (handle) and following (treated) shoot organogenesis induction. Bubble size and color indicate the frequency with the GO term as well as the PRMT1 Storage & Stability P-value, respectively.Frontiers in Plant Science | frontiersin.orgAugust 2021 | Volume 12 | ArticleTorres-Silva et al.De novo Transcriptome of M. glaucescens Shoot OrganogenesisTABLE 3 | Transcription issue families containing downregulated and upregulated unigenes following M. glaucescens shoot organogenesis induction. Transcription aspect household Domain No. of unigenes Downregulated Upregulated AP2 AP2 zf-Dof DUF260 B3 and auxin response B3 zf-LSD1 G2-like GRAS Homeobox Homeobox zf-CCCH NAM bZIP_1 WRKY Myb_DNATRPML Storage & Stability -binding Myb_DNA-binding HLH EIN3 HSF_DNA-bind zf-C2H2 Trihelix GATA two 1 0 5 1 0 two 2 1 0 4 0 three 1 4 0 1 0 4 0 1 three 0 0 0 0 0 1 3 1 0 0 0 1 0 1 1 five 1 7 0 4 11 5 two 2 three 3 1 five three 17 5 two 10 four four 3 2 7 eight 5 two 2 1 1 0 1 0 1 1 1 1 1 0 0 0ERF loved ones protein AP2 family members protein Dof household protein LBD family protein ARF loved ones protein B3 family protein LSD family protein G2-like family members protein GRAS family protein HB-other loved ones protein TALE household protein C3H household protein NAC household protein bZIP household protein WRKY family members protein MYB loved ones protein MYB_related loved ones protein bHLH family protein EIL family members protein HSF family members protein C2H2 household protein Trihelix household protein GATA household proteinHD-ZIP loved ones protein HomeoboxE2F/DP family protein E2F_TDP CAMTA loved ones protein CG-1 NF-YC family members protein NF-YA family protein NF-X1 loved ones protein ZF-HD household protein SBP family protein YABBY loved ones protein FAR1 household protein MIKC_MADS family protein GRF family protein S1Fa-like loved ones protein TCP household protein DBB family members protein NF-YC CBFB_NFYA zf-NF-X1 ZF-HD_dimer SBP YABBY FAR1 SRF-TF and K-box WRC and QLQ S1FA TCP zf-B_boxCO-like family members protein zf-B_box and CCTdivision and cell wall formation through regeneration (Bao et al., 2009). Furthermore, modifications in cell fate can take place as a result of a brand new balance in between euchromatin and heterochromatin following dynamic alterations in chromatin structure (Zhao et al., 2008), indicated right here by the presenceof histone-encoding genes among the upregulated transcripts (Figure five). Transcription issue households and unigenes were additional abundant among the upregulated than downregulated unigenes (Table three). This finding indicates that numerous metabolic pathways are activated during shoot organogenesis induction. The families of transcription components found to be upregulated in this study (i.e., NF-Y, MYB, ERF, E2F, LBD, and NAC) are akin to those identified through plant organogenesis, additional confirming the role of these regulators in vegetative regeneration (Cheong et al., 2002; Bao et al., 2009; Cervantes-P ez et al., 2018; Nadiya et al., 2018; Pal et al., 2018; Xi et al., 2019). Through the in vitro culture preceding shoot organogenesis induction, the metabolism of M. glaucescens was characterized by fewer upregulated than downregulated genes, corroborating the low growth rate common of cacti cultures (Lema-Rumiska and n Kulus, 2014; P ez-Molphe-Balch et al., 2015). Downregulated genes had been largely involved in primary metabolism. They incorporated transcripts connected to photosynthesis (15) and antenna proteins (five) identified in KEGG
