The catalytic catalytic abilityas a substrate substrate the above the above results. 3 sorts of the ability with N with N as a determined by determined by benefits. Three types of media (which includes LB, TB and M9) andand M9) and five substrate concentrations for this study for media (such as LB, TB 5 substrate concentrations had been chosen were selected (Figure 5). The outcomes showed that the best substratethe ideal substrate 80 mg-1, and was L this study (Figure five). The results showed that 5-HT2 Receptor Antagonist Purity & Documentation concentration was concentration the optimal L-1 , along with the E production wasfor E The highest was M9. The highest of E of 80 mgmedium for optimal medium M9. production conversion efficiency conversion the P2-carryingof E of was P2-carrying strain was 39.58 L-1), with a final substrate oncen- a efficiency strain the 39.58 3.six (31.67 two.89 mg three.6 (31.67 2.89 mg L-1 ), with tration of substrate concentration of 80 mg -1 inthat medium, followed by 2.52 mg edium L final 80 mg-1 in M9 medium, followed by M9 in TB medium (27.87 that in TB L-1), (27.87 in LB mg -1 whilst that in LB medium was theL-1). Probably the most exciting -1 ). though that 2.52 medium),was the lowest (22.72 1.14 mg owest (22.72 1.14 mgresult One of the most exciting outcome efficiency of E produced by the P2 3-carrying by the P2 3-carrying was that the conversion was that the conversion efficiency of E producedstrain in the constrain in the conversion efficiency 2.85 mg-1). Therefore, M9 medium and M9 medium version efficiency was up to (46.84 was as much as (46.84 2.85 mg -1 ). Hence,80 mg-1 N and L L were80 mg -1 thewere chosen as theand substrate concentration, respectively, for the subchosen as N optimal medium optimal medium and substrate concentration, respectively, for study. sequent the subsequent study.Molecules 2021, 26, FOR Molecules 2021, 26, x 2919 PEER REVIEW8 13 eight ofofFigure Conversion efficiency of E in distinct media (LB, TB and M9) and substrate concentrations Figure 5.5. Conversion efficiency of E in differentmedia (LB, TB and M9) and substrate concentra(substrate concentrations from 40 40 L-1L-1 120 mg – ). (a): the conversion efficiency of E of tions (substrate concentrations frommg gto to 120 mg1-1).(a): the conversion efficiency of E on the L the P2-carrying strain in LB, TB and M9 media. (b): the conversion efficiency of E from the P2 3P2-carrying strain in LB, TB and M9 media. (b): the conversion efficiency of E from the P2 3-carrying carryingin LB, TB and TB and M9 media. Data are because the indicates s.d.s s.d.s (n = three). strain strain in LB, M9 media. Information are shown shown as the suggests (n = 3).three.4. Substrate Diversity Evaluation the HpaBC Complex 3.4. Substrate Diversity Analysis ofof the HpaBC Complex To additional investigate diversity of substrates, in addition to flavanone (N), a (N), To additional investigate thethe diversity of substrates, as well as flavanone mon- a monohydroxylated phenolic (p-coumaric acid, p-CA), AT1 Receptor Antagonist MedChemExpress dihydroflavonol (DHK), flavonol ohydroxylated phenolic acid acid (p-coumaric acid, p-CA), dihydroflavonol (DHK), flavonol (K), flavan-3-ol (afzelechin, Af) and anthocyanin (pelargonidin, PEL) have been fed under the (K), flavan-3-ol (afzelechin, Af) and anthocyanin (pelargonidin, PEL) were fed below the optimal situations, plus the fermentation products were detected by HPLC and LC-MS optimal conditions, along with the fermentation solutions were detected by HPLC and LC-MS procedures (Figure 6). Preceding research have suggested that the HpaBC complicated has in vivo approaches (Figure six). Earlier studies have.
