Published version from the manuscript. Funding: This investigation was funded by
Published version in the manuscript. Funding: This research was funded by Daugavpils University internal grant No. 14-95/2021/8. Institutional Review Board Statement: Not applicable. Informed Consent Statement: Not applicable. Information Availability Statement: The data presented in this study are out there on request from the corresponding author. Acknowledgments: The authors are thankful to chem. Ligita Mezaraupe for helping with confocal laser scanning microscopy. Conflicts of Interest: The authors declare no conflict of interest.
moleculesArticleComparison of Different d-SPE Sorbent Performances Based on Swift, Straightforward, Cheap, Successful, Rugged, and Protected (QuEChERS) Methodology for Multiresidue Pesticide Analyses in RapeseedsSaida Belarbi 1,two, , Martin Vivier two , Wafa Zaghouani two , Aude De Sloovere two , Valerie Agasse 1 and Pascal Cardinael 1, Laboratoire SMS-EA3233, Normandie University, FR3038 INC3M, Unirouen, Spot Emile Blondel, F-76821 Mont-Saint-Aignan, France; [email protected] SGS France Laboratoire de Rouen, Technop e du Madrillet, 65 Avenue Ettore Bugatti, BP 90014, F-76801 Saint Etienne du Rouvray, France; [email protected] (M.V.); [email protected] (W.Z.); [email protected] (A.D.S.) Correspondence: [email protected] (S.B.); [email protected] (P.C.); Tel.: 33-235522960 (P.C.)Citation: Belarbi, S.; Vivier, M.; Zaghouani, W.; De Sloovere, A.; Agasse, V.; Cardinael, P. Comparison of Various d-SPE Sorbent Performances Based on Speedy, Quick, Inexpensive, Effective, Rugged, and Secure (QuEChERS) Methodology for Multiresidue Pesticide Analyses in Rapeseeds. Molecules 2021, 26, 6727. https://doi.org/10.3390/ molecules26216727 Academic Editors: Angelina Lopes Simoes Pena and Sofia Cancela Duarte Received: 22 October 2021 Accepted: 3 November 2021 Published: 6 NovemberAbstract: Pesticide extraction in rapeseed samples remains a terrific analytical challenge because of the complexity with the matrix, which includes proteins, fatty acids, high amounts of triglycerides and cellulosic PHA-543613 Epigenetic Reader Domain fibers. An HPLC-MS/MS approach was created for the quantification of 179 pesticides in rapeseeds. The performances with the speedy, uncomplicated, low-cost, powerful, rugged, and safe (QuEChERS) approach have been evaluated working with distinct dispersive solid-phase extraction (d-SPE) sorbents containing common octadecylsilane silica/primary econdary amine adsorbent (PSA/C18) and new commercialized d-SPE materials dedicated to fatty matrices (Z-Sep, Z-Sep , and EMR-Lipid). The analytical performances of these various sorbents were compared as outlined by the SANTE/12682/2019 document. The best final results have been obtained applying EMR-Lipid in terms of pesticide average recoveries (103 and 70 from the 179 targeted pesticides exhibited recoveries within 7020 and 300 , respectively, with low RSD values). In addition, the limits of quantification (LOQ) variety from 1.72 /kg to six.39 /kg for 173 in the pesticides. Only the recovery for tralkoxydim at 10 /kg level was not satisfactory (29 ). The Goralatide supplier matrix effect was evaluated and proved to become limited between -50 and 50 for 169 pesticides with this EMR-Lipid and freezing. GC-Orbitrap analyses confirmed the most effective efficiency on the EMR-Lipid sorbent for the purification of rapeseeds. Keywords: multiresidue pesticide analyses; rapeseeds; QuEChERS extraction; dispersive solid-phase extraction; HPLC-MS/MSPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.1. Introductio.
