-old, randomly selected in an urban community located in the periphery of the city of Salvador, Brazil and followed them from January/2008 to January/2009. Nasopharyngeal swabs were collected from each child at four times. In total, 721 swabs were collected, yielding a pneumococcal carriage NSC 697286 site prevalence of 55 (n=398). In Pan-RAS-IN-1 site multivariate analyses, the variables associated with carriage were having contact with three or more children <2 years old (OR, 2.00; 95 CI 1.33?.89) and living in a house with an average of 3 residents per room (OR, 1.77; 95 CI 1.05?.10). Also, white participants were more likely to be protected from colonization (OR, 0.52; 95 CI 0.29?.93), and prevalence of carriage varied over time, with?Corresponding author: Joice Neves Reis, Ph.D.; Departamento de An ises Cl icas e Toxicol icas, Faculdade de Farm ia, Universidade Federal da Bahia, 40170115 Salvador, BA, Brasil. [email protected]. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Disclaimers: The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the Centers for Disease Control and Prevention or the institutions with which the authors are affiliated.Author Contributions Conceived and designed the experiments: JNR, AK, MGC. Performed the experiments: APOM, MGC. Collect the samples: APOM, JA, LCC, MCL, JNR. Analyzed the data: APOM, MC,DW, GR. Contributed reagents/materials/analysis tools: JNR, MR, MGC. Wrote the paper: JNR, APOM, GR, DW. Reviewed and revised the final version of the manuscript: All authors.Menezes et al.Pagelower prevalence occurring from February to June (OR, 0.53; 95 CI 0.37?.78) compared to July to January. Contact with children under two years of age and living in crowded housing also were associated with colonization by highly invasive serotypes, although this relationship was not significant. The most prevalent vaccine serotypes were 6A/B (25.4 ), 19F (10.1 ) and 14 (9.0 ), while the most prevalent non-vaccine serotypes were 16F (4.8 ), 15B/C (4.5 ) and 6C/D (3.5 ). Overall, 38.4 (153/398) of the isolates were non-susceptible to penicillin, and of those, 73.8 (113/153) were non-susceptible to trimethoprim/sulfamethoxazole. Colonization rate by PCV10 serotypes was 52.2 . Routine PCV10 vaccination can lead to significant changes in pneumococcal serotypes found in NP colonization, indicating a need for continued monitoring, especially in crowded settings, as occurs in Brazil's slums.Author Manuscript Author Manuscript Author Manuscript Author ManuscriptKeywords Children; nasopharyngeal carriage; Streptococcus pneumoniae; serotypes; PCV10-vaccineBackgroundAsymptomatic carriage of pneumococci is common in young children and has been related to the development of disease and transmission of the pathogen [1?]. The prevalence of pneumococcal carriage increases in the first few years of life, peaking at approximately 50 to 80 in children 2? years of age and decreasing thereafter until stabilizing at 5 to 10 in children over 10 years of age [4]. Effective vaccin.-old, randomly selected in an urban community located in the periphery of the city of Salvador, Brazil and followed them from January/2008 to January/2009. Nasopharyngeal swabs were collected from each child at four times. In total, 721 swabs were collected, yielding a pneumococcal carriage prevalence of 55 (n=398). In multivariate analyses, the variables associated with carriage were having contact with three or more children <2 years old (OR, 2.00; 95 CI 1.33?.89) and living in a house with an average of 3 residents per room (OR, 1.77; 95 CI 1.05?.10). Also, white participants were more likely to be protected from colonization (OR, 0.52; 95 CI 0.29?.93), and prevalence of carriage varied over time, with?Corresponding author: Joice Neves Reis, Ph.D.; Departamento de An ises Cl icas e Toxicol icas, Faculdade de Farm ia, Universidade Federal da Bahia, 40170115 Salvador, BA, Brasil. [email protected]. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Disclaimers: The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the Centers for Disease Control and Prevention or the institutions with which the authors are affiliated.Author Contributions Conceived and designed the experiments: JNR, AK, MGC. Performed the experiments: APOM, MGC. Collect the samples: APOM, JA, LCC, MCL, JNR. Analyzed the data: APOM, MC,DW, GR. Contributed reagents/materials/analysis tools: JNR, MR, MGC. Wrote the paper: JNR, APOM, GR, DW. Reviewed and revised the final version of the manuscript: All authors.Menezes et al.Pagelower prevalence occurring from February to June (OR, 0.53; 95 CI 0.37?.78) compared to July to January. Contact with children under two years of age and living in crowded housing also were associated with colonization by highly invasive serotypes, although this relationship was not significant. The most prevalent vaccine serotypes were 6A/B (25.4 ), 19F (10.1 ) and 14 (9.0 ), while the most prevalent non-vaccine serotypes were 16F (4.8 ), 15B/C (4.5 ) and 6C/D (3.5 ). Overall, 38.4 (153/398) of the isolates were non-susceptible to penicillin, and of those, 73.8 (113/153) were non-susceptible to trimethoprim/sulfamethoxazole. Colonization rate by PCV10 serotypes was 52.2 . Routine PCV10 vaccination can lead to significant changes in pneumococcal serotypes found in NP colonization, indicating a need for continued monitoring, especially in crowded settings, as occurs in Brazil's slums.Author Manuscript Author Manuscript Author Manuscript Author ManuscriptKeywords Children; nasopharyngeal carriage; Streptococcus pneumoniae; serotypes; PCV10-vaccineBackgroundAsymptomatic carriage of pneumococci is common in young children and has been related to the development of disease and transmission of the pathogen [1?]. The prevalence of pneumococcal carriage increases in the first few years of life, peaking at approximately 50 to 80 in children 2? years of age and decreasing thereafter until stabilizing at 5 to 10 in children over 10 years of age [4]. Effective vaccin.
