Diac dysfunction and the pathological 12 / 18 Exercise and Myocardial Infarction in OVX Rats Fig. 6. Myocyte cross sectional area evaluation. Representative images of histological sections stained with hematoxylin and eosin of Control, OVX+SHAMSED, OVX+SHAMET, OVX+MISED and OVX+MIET groups. Data are expressed as mean SEM. P,0.05. Magnifier 400x. Bar: 50 mm. doi:10.1371/journal.pone.0115970.g006 remodeling process in ovariectomized rats after MI, investigating the possible mechanisms involved in these processes. The present study has three major findings: i) ET improved the parameters of cardiac function in ovariectomized rats after MI; ii) ET attenuated the effects of MI-induced remodeling; and iii) ET decreased the protein expression of one of the main pathways generating reactive oxygen species and also increased the antioxidant enzyme catalase, which contributes to both improved cardiac function and to the remodeling process. The enhancement of collagen deposition plays an important role in adverse remodeling after MI. In our study, the animals subjected to eight weeks of ET showed a reduction in collagen deposition compared to the sedentary group. A mechanism that may explain the beneficial effects of ET after MI is the reduction in RAAS activation. The neurohumoral cascade after ischemic cardiac events 13 / 18 Exercise and Myocardial Infarction in OVX Rats increases the production of AngII by fibroblasts. The effects of AngII PubMed ID:http://jpet.aspetjournals.org/content/12/4/255 are exerted by the activation of two receptor subtypes where the effects of AT1 Tauroursodeoxycholic acid sodium salt subtype predominate over AT2. Once activated in cardiac cells, the AT1 receptor causes an increase in collagen deposition via multiple signaling pathways. These effects may be exacerbated in the setting of ovarian hormone deficiency, as is the case in postmenopausal women. Pedram et al., showed that reduction in circulating estrogen levels increases AngII and endothelin-1 production by fibroblasts, macrophages and the endothelium. Their actions mediate transforming growth factor b which stimulates both matrix metalloproteinase production and the modification of fibroblasts into myofibroblasts, a process that culminates in the synthesis of collagen types I and III. It is noteworthy that the normal adult heart is composed of approximately 2 to 4 collagen, the presence of which confers high tensile strength, and slight changes in the heart’s composition may Rocaglamide adversely affect cardiac contractility; therefore, the higher the collagen concentration, the worse the contractile force exerted by the myocardium. A study conducted by Wenhan Wan et al, evaluated how ET attenuates RAAS activation and the subsequent remodeling process after MI. They showed that ET reduces circulating levels of renin and angiotensin converting enzyme as well as plasmatic concentrations of AngII and aldosterone, which are associated with the preservation of cardiac function. These effects are independent of the time the training starts. Similarly, Braith et al., demonstrated that 16 weeks of training decreases circulating levels of AngII in patients with heart failure after MI. It is important to note that although we didn’t evaluate the various components of RAAS, the reduction in AT1 receptor expression suggests that ET reduces collagen deposition via this process. As demonstrated in our study, the 
increase in collagen deposition in MI animals was accompanied by the reduction of both contraction force as well as an increase in LVEDP, as described by others. In an.Diac dysfunction and the pathological 12 / 18 Exercise and Myocardial Infarction in OVX Rats Fig. 6. Myocyte cross sectional area evaluation. Representative images of histological sections stained with hematoxylin and eosin of Control, OVX+SHAMSED, OVX+SHAMET, OVX+MISED and OVX+MIET groups. Data are expressed as mean SEM. P,0.05. Magnifier 400x. Bar: 50 mm. doi:10.1371/journal.pone.0115970.g006 remodeling process in ovariectomized rats after MI, investigating the possible mechanisms involved in these processes. The present study has three major findings: i) ET improved the parameters of cardiac function in ovariectomized rats after MI; ii) ET attenuated the effects of MI-induced remodeling; and iii) ET decreased the protein expression of one of the main pathways generating reactive oxygen species and also increased the antioxidant enzyme catalase, which contributes to both improved cardiac function and to the remodeling process. The enhancement of collagen deposition plays an important role in adverse remodeling after MI. In our study, the animals subjected to eight weeks of ET showed a reduction in collagen deposition compared to the sedentary group. A mechanism that may explain the beneficial effects of ET after MI is the reduction in RAAS activation. The neurohumoral cascade after ischemic cardiac events 13 / 18 Exercise and Myocardial Infarction in OVX Rats increases the production of AngII by fibroblasts. The effects of AngII PubMed ID:http://jpet.aspetjournals.org/content/12/4/255 are exerted by the activation of two receptor subtypes where the effects of AT1 subtype predominate over AT2. Once activated in cardiac cells, the AT1 receptor causes an increase in collagen deposition via multiple signaling pathways. These effects may be exacerbated in the setting of ovarian hormone deficiency, as is the case in postmenopausal women. Pedram et al., showed that reduction in circulating estrogen levels increases AngII and endothelin-1 production by fibroblasts, macrophages and the endothelium. Their actions mediate transforming growth factor b which stimulates both matrix metalloproteinase production and the modification of fibroblasts into myofibroblasts, a process that culminates in the synthesis of collagen types I and III. It is noteworthy that the normal adult heart is composed of approximately 2 to 4 collagen, the presence of which confers high tensile strength, and slight changes in the heart’s composition may adversely affect cardiac contractility; therefore, the higher the collagen concentration, the worse the contractile force exerted by the myocardium. A study conducted by Wenhan Wan et al, evaluated how ET attenuates RAAS activation and the subsequent remodeling process after MI. They showed that ET reduces circulating levels of 
renin and angiotensin converting enzyme as well as plasmatic concentrations of AngII and aldosterone, which are associated with the preservation of cardiac function. These effects are independent of the time the training starts. Similarly, Braith et al., demonstrated that 16 weeks of training decreases circulating levels of AngII in patients with heart failure after MI. It is important to note that although we didn’t evaluate the various components of RAAS, the reduction in AT1 receptor expression suggests that ET reduces collagen deposition via this process. As demonstrated in our study, the increase in collagen deposition in MI animals was accompanied by the reduction of both contraction force as well as an increase in LVEDP, as described by others. In an.
