atory by specific commercial sandwich enzyme-linked immunosorbent assays. The tests were quantified at 450 nm in a dual-wavelength microplate reader using Magellan software. The sTNF-R1 and IL-6 tests have limits of detection of 25 and 0.3 pg/ml, respectively. Our intra-assay and inter-assay coefficients of variation were 6.5 and 9.1% for sTNF-R1 and 6.1 PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/22180813 and 8.3% for IL-6, respectively. Echo-Doppler study The study was performed using standard hospital echocardiographic systems equipped with 2.54 MHz transducers. The Long-Term Variation of PF-04447943 NT-proBNP in Hypertension echocardiographic examinations were performed using the standard apical and parasternal long axis views. Doppler echocardiogram images were stored on videotape and analyses of recordings were performed in a central laboratory. M-Mode and two-dimensional images, Doppler spectrum and color Doppler were analyzed off-line. For each patient, four consecutive beats were measured and averaged for each Doppler variable. To obtain left ventricular ejection fraction, the area-length method was used. Left ventricular mass was measured following the Devereux method and in our study LVH was defined as.46.7 g/m2.7 in women and.49.2 g/m2.7 in men. The following measurements were used for assessment of LV diastolic dysfunction: 1) mitral flow propagation velocity was determined using the previously described method; 2) peak flow velocity in early diastole and during atrial contraction was measured by pulsed Doppler at valve level, calculating the E/A ratio; 3) early LV filling deceleration time was measured as the distance between the projection of the peak velocity on the baseline and the point where EF slope encounters the baseline; 4) the inclination of the straight line of the ascending mitral ring in M-mode recording shows maximum ascending velocity of the mitral annulus during early diastole. The maximum relaxation velocity was calculated as mean value of the maximal velocities in the septal, lateral, posterior, and anterior portion of the annulus. Intra-observer variability was consecutively evaluated in series of 40 patients. Variability was expressed as the absolute difference divided by the mean value of echocardiographic measurements, left ventricular mass variability being 8.466%. approach, the limits of agreement were determined by the mean difference plus or minus the coefficient of reproducibility, where CR was calculated as 1.966 SD of the percentage of changes. In this case, a high CR indicates poor reproducibility. The coefficient of variation is calculated following CV = 100. The total CV and the analytical CV provided the basis for the individual biological CV following CVi = 1/2. Reference change values were calculated from median CVt values, according to the formula RCV = Z621/2 1/2, where 
Z = 1.96; na is the number of replicate assays; and ns is the number of patient samples to estimate each of the two homeaostatic set points. A p value,0.05 was considered significant for all measures. All statistical analyses were performed using the SPSS statistical software package. Results The baseline characteristics and natriuretic peptide serum levels of the hypertensive patients in the three stages according to hypertrophy are shown in Statistical analyses Continuous variables are presented as mean 6 standard deviation and categorical variables as a number of patients or percentage. Results for each variable were tested for normality using the Kolmogorov Smirnov method. NT-proB
