Ub aldehyde
Ub aldehyde is a potent and highly specific inhibitor of cysteine deubiquitinating enzymes including the Ub C-terminal hydrolases (UCHs), the Ub-specific proteases (USPs), the ovarian tumor domains (OTUs) proteases and the Machado-Josephine domain (MJD) proteases. Typical working concentration is 2-5 µM in vitro.
Product Name:
Ub aldehyde
>Also Known As:
Ubiquitin aldehyde, Ubal
>Catalog No.:
F3100
>Size:
50 µg
>Molecular Weight:
9 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
20 mM sodium acetate, pH 5.0
>Concentration:
See tube label
>Quality Assurance:
~95% by SDS-PAGE, see datasheet for gel image
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
N/A
>Image(s):
Click image to enlarge
Open circle: 250 nM Ub-Rhodamine 110
Solid triangle: 250 nM Ub-Rhodamine 110 + 5 nM GST-USP4
Solid circle: 250 nM Ub-Rhodamine 110 + 5 nM GST-USP4 + 2 μM Ubal (overlapped with open circles)
Reaction buffer: 40 mM Tris, pH 7.1 at 37°C, 150 mM NaCl, 5 mM DTT and 10% glycerol
>Shipping Method:
Dry ice shipping
>References:
1. Hershko A,et al. (1987) Proc Natl Acad Sci 84, 1829 – 1833.
2. Hadari T, et al. (1992) J Biol Chem 267(2), 719 – 727.
3. Melandri F, et all. (1996 Biochemistry 35(39), 12893 – 12900.
4. Hu, M. et al. (2005) EMBO J. 24. 3747. ALW-II-41-27 18469924
