Ub-AMC
Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.
Product Name:
Ub-AMC
>Also Known As:
Ubiquitin-AMC
>Catalog No.:
M3010
>Size:
50 µg
>Molecular Weight:
9 kDa
>Species:
Human
>Source:
Bacterial recombinant
>Stock:
DMSO
>Concentration:
See tube label
>Quality Assurance:
>95% by SDS-PAGE
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet
>NCBI RefSeq:
N/A
>Image(s):
No
>Shipping Method:
Dry ice shipping
>References:
Wilkinson KD, et al. (2005) Methods Enzymol. 399, 37-51 BLZ945 18469813
