TAMRA-Lys(Ub)-Gly
This products is a fluorescence polarization (FP) assay reagent, which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitine via a native isopeptide bond with the lysine side-chain. Typical substrate concentrations range from 10−100 nM. DUB concentrations can range from 0.01-10 nM but depend on specific assay conditions and method of detection. FP assays can be performed with any fluorescence polarization reader with a 530 nm excitation filter and two 580 nm emission filters.
Product Name:
TAMRA-Lys(Ub)-Gly
>Also Known As:
N/A
>Catalog No.:
M3030
>Size:
50 µg
>Molecular Weight:
9163 Da by MS
>Species:
Human
>Source:
Synthetic
>Stock:
Product in lyophilized powder form.
>Concentration:
N/A
>Quality Assurance:
≥95% by RP-HPLC and SDS-PAGE
>Storage:
Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles.
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
N/A
>Image(s):
(Click image to enlarge)
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>Shipping Method:
Room temperature shipping
>References:
1. Tirat, A. et al., (2005) Anal. Biochem. 343, 244.
2. Huang et al., (2009) Methods in Molecular Biology 565, 127.
3. Levine et al., (1997) Anal. Biochem. 247, 83.
4. Geurink and El Oualid et al., (2012) ChemBiochem 13, 293. MK-0974 18470564
