Rapid 26S Proteasome Purification Kit-S
The Rapid 26S Proteasome Purification Kit is designed to rapidly purify the endogenous 26S proteasome from cell or tissue extracts within 8 hours according to a published method (reference 1) with modifications. This approach uses the N-terminal ubiquitin-like (Ubl) domain of human RAD23B as an affinity bait, which allows the rapid and gentle isolation of endogenous 26S proteasomes. The bound 26S proteasome is subsequently eluted using the C-terminal two ubiquitin-interacting motifs (UIM) of human S5a.
Product Name:
Rapid 26S Proteasome Purification Kit-S
>Also Known As:
N/A
>Catalog No.:
J4310
>Size:
1 Kit
>Molecular Weight:
N/A
>Species:
N/A
>Source:
N/A
>Stock:
Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol; Substrate stocked in DMSO
>Concentration:
See tube label
>Quality Assurance:
Enzyme >95% by SDS-PAGE; Substrate > 98% by HPLC and NMR
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF datasheet, MSDS
>NCBI RefSeq:
N/A
>Image(s):
(Click image to enlarge)
|
Schematic of 26S proteasome enrichment |
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>Shipping Method:
Dry ice shipping
>References:
1. Besche HC, Haas W, Gygi SP, Goldberg AL. Biochemistry. 2009; 48:2538-49.
2. Elsasser S, Schmidt M, Finley D. Methods Enzymol. 2005; 398:353-63.
3. Kisselev AF, Goldberg AL. Methods Enzymol. 2005; 398:364-78.
5-Iodotubercidin 18485237
