N-terminal Biotin-Ubiquitin
A single biotin moiety is conjugated on the N-terminus of ubiquitin. All lysine residues of ubiquitin are still available for formation of polyubiquitin chains. The extremely high binding affinity between biotin and streptavidin allows the use of N-terminal biotin-ubiquitin for the following in vitro assays: 1) rapid and efficient purification of polyubiquitinated substrate proteins; 2) monitoring substrate ubiquitination using an HRP-conjugated streptavidin antibody; and 3) setting up high throughput FRET assays to monitor substrate ubiquitination in assays screening E3 ubiquitin ligase inhibitors.
Product Name:
N-terminal Biotin-Ubiquitin
>Also Known As:
Biotin-Ub
>Catalog No.:
E1140
>Size:
50 µg
>Molecular Weight:
8.9 kDa
>Species:
Human
>Source:
Synthetic
>Stock:
20 mM Tris, pH 7.6 at 4 °C, 100 mM NaCl, 10% glycerol
>Concentration:
1 mg/ml in aqueous buffer
>Quality Assurance:
> 95% by HPLC
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF Datasheet， MSDS
>NCBI RefSeq:
N/A
>Image(s):
(Click image to enlarge)
 |
| LC-MS of N-terminal Biotin-Ubiquitin |
>Shipping Method:
Dry ice shipping
>References:
N/A Trametinib 18476228
