K48 Polyubiquitin Chain Capture Kit
This kit is designed for enrichment of cellular proteins conjugated with lysine 48-linked polyubiquitin chains using GST-S5a (UIM). Polyubiquitinated proteins bound on GST-S5a (UIM) can be precipitated using gluta-thione resin and eluted by a buffer containing 10 mM glutathione. Polyubiquitinated proteins can be as-sayed by immunoblotting or mass spectrometry.
Product Name:
K48 Polyubiquitin Chain Capture Kit
>Also Known As:
N/A
>Catalog No.:
J4430
>Size:
1 Kit
>Molecular Weight:
N/A
>Species:
N/A
>Source:
N/A
>Stock:
Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
>Concentration:
See tube label
>Quality Assurance:
Enzyme >95% by SDS-PAGE
>Storage:
Store at -80°C; avoid multiple freeze-thaw cycles
>PDF Data Sheet:
PDF datasheet, MSDS
>NCBI RefSeq:
N/A
>Image(s):
(Click image to enlarge)
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Schematic of polyUb chains enrichment |
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Lane 1: GST Lane 2: GST-S5a (UIM) Lane 3: GST-TAB2 (NZF) Lane 4: GST-S5a (UIM) + GST-TAB2 (NZF) Lane 5: GST-NEMO (UBAN) According to procedures described above, 5 mg whole cell lysates from HeLa cells were mixed with 0.2 mg GST (lane 1), GST-S5a (UIM) (lane 2), GST-TAB2 (NZF) (lane 3), GST-S5a (UIM) + GST-TAB2 (NZF) (lane 4), or GST-NEMO (UBAN) (lane 5). 10% eluates were separated by SDS-PAGE, followed by immunoblotting of Ub, K48-specific Ub chains and GST. * No immunoreactivity was detected in GST-NEMO (UBAN) pulldown assays, presumably because there is little linear polyubiquitin chains in cells. |
>Shipping Method:
Dry ice shipping
>References:
N/A BMS-626529 18483682
